Abstract | BACKGROUND: METHODS: Primary cultures of NPFs were established from nasal polyps (NPs). Productions of Cyr61, VEGF, and IL-8 by NPFs under hypoxia were detected by Western blot (Cyr61 and VEGF) or enzyme-linked immunosorbent assay (ELISA; IL-8). Immunohistochemical staining was used to examine the relation between fibroblastic expression of Cyr61 and neovascularization/neutrophil infiltration in NPs. RESULTS: Western blot showed that the hypoxia inducer CoCl(2) stimulated Cyr61 synthesis in NPFs in a time-dependent manner, reaching a peak at 24 hours. Bay-117082 (a specific NF-kappaB inhibitor) attenuated the levels of Cyr61 stimulated by hypoxia. Cyr61 induced IL-8 secretion and VEGF synthesis by NPFs, as evidenced by Western blot and ELISA analysis. Bay-117082 abolished hypoxia-stimulated IL-8 and VEGF synthesis, whereas Cyr61 restored the stimulative effect of hypoxia readily. Immunohistochemical staining revealed the presence of Cyr61 and IL-8 in NPFs. Neutrophils and capillaries aggregating around these NPFs were frequently found. CONCLUSION: Under hypoxia, NPFs contribute to NP propagation by expressing Cyr61, which subsequently stimulates VEGF and IL-8 production, leading to angiogenesis and activating neutrophil infiltration in NPs.
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Authors | Chia-Tung Shun, Sze-Kwan Lin, Chi-Yuan Hong, Hung-Meng Huang, Chia-Ming Liu |
Journal | American journal of rhinology & allergy
(Am J Rhinol Allergy)
2011 Jan-Feb
Vol. 25
Issue 1
Pg. 15-8
ISSN: 1945-8932 [Electronic] United States |
PMID | 21711965
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 3-(4-methylphenylsulfonyl)-2-propenenitrile
- Cysteine-Rich Protein 61
- Interleukin-8
- NF-kappa B
- Nitriles
- Sulfones
- Vascular Endothelial Growth Factor A
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Topics |
- Cell Movement
- Cells, Cultured
- Chronic Disease
- Cysteine-Rich Protein 61
(genetics, metabolism)
- Fibroblasts
(drug effects, metabolism, pathology)
- Humans
- Hypoxia
(pathology, physiopathology)
- Interleukin-8
(genetics, metabolism)
- NF-kappa B
(antagonists & inhibitors)
- Nasal Mucosa
(blood supply, metabolism, pathology)
- Nasal Polyps
- Neovascularization, Pathologic
- Neutrophils
(immunology)
- Nitriles
(pharmacology)
- Rhinitis
(genetics, metabolism, pathology, physiopathology)
- Sinusitis
(genetics, metabolism, pathology, physiopathology)
- Sulfones
(pharmacology)
- Vascular Endothelial Growth Factor A
(genetics, metabolism)
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