Inflammation-related signaling pathways implicating TGFβ are revealed in the expression profiling of MCF7 cell treated with fermented soybean, chungkookjang.

Abstract	Chungkookjang is a Korean fermented soybean containing microorganisms, proteinase, and diverse bioactive compounds, including a high concentration of isoflavones and peptides. Growth of breast cancer MCF7 cells decreased dependent on the concentration of fermented soybean extracts. The effect of fermented soybean on cellular gene expression was determined in a systematic manner comprehensively. DNA microarray analysis was performed using 25,804 probes. Ninety one genes whose expression levels were significantly changed were selected. TGFβI and Smad3 were upregulated. Downregulation of inflammation-related CSF2, CSF2RA, and CSF3 was found. Differential expression of chemokines CCL2, CCL3, CCL3L3, CXCL1, and CXCL2 were observed. Network analysis identified ERβ in the network. Based on the experimental results, taking fermented soybean might be helpful for preventing breast cancer by a mechanism activating TGFβ pathway and depressing inflammation.
Authors	Jae Sung Hwang, Hyung Jae Yoo, Hye Jung Song, Kyu Kwang Kim, Young Jin Chun, Toshiro Matsui, Han Bok Kim
Journal	Nutrition and cancer (Nutr Cancer) Vol. 63 Issue 4 Pg. 645-52 ( 2011) ISSN: 1532-7914 [Electronic] United States
PMID	21547849 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	CSF2RA protein, human CSF3R protein, human Chemokines Isoflavones Receptors, Colony-Stimulating Factor Receptors, Granulocyte-Macrophage Colony-Stimulating Factor SMAD3 protein, human Smad3 Protein Soybean Proteins Transforming Growth Factor beta chungkookjang
Topics	Cell Line, Tumor Cell Survival Chemokines (genetics, metabolism) Down-Regulation Fermentation Gene Expression Profiling Humans Isoflavones (pharmacology) Oligonucleotide Array Sequence Analysis Receptors, Colony-Stimulating Factor (genetics, metabolism) Receptors, Granulocyte-Macrophage Colony-Stimulating Factor (genetics, metabolism) Signal Transduction Smad3 Protein (genetics, metabolism) Soybean Proteins (pharmacology) Soybeans Transforming Growth Factor beta (genetics, metabolism) Up-Regulation

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