A Phellinus baumii extract reduces obesity in high-fat diet-fed mice and absorption of triglyceride in lipid-loaded mice.

Abstract

This study evaluated the anti-obesity effects of Phellinus baumii extract (PBE) in high-fat diet (HFD)-fed mice. Male 8-week-old C57BL/6 mice were randomly divided into four groups: control, normal chow diet plus vehicle; HFD-control, high-fat plus vehicle; HFD plus orlistat (Xenical(®), Roche, Basel, Switzerland) (50 mg/kg); and HFD plus PBE (500 mg/kg). PBE was administered daily by oral gavage for 12 weeks. Oral administration of PBE (500 mg/kg) significantly reduced body weight gain, hepatic lipid concentrations, and fat accumulation in epididymal adipocytes compared with mice fed HFD alone (P < .05). mRNA expression of genes related to triglyceride (TG) synthesis was suppressed in the PBE groups, and fatty acid synthase activity was also significantly inhibited (P < .05). Furthermore, we evaluated the effect of PBE on TG absorption and detected marked reduction in TG absorption in Xenical- and PBE-treated mice compared with the control group (P < .05). To determine the active compound of PBE, fractionation was conducted, and interfungin A, davallialactone, and hypholomine B were identified as the main compounds. Among the three identified compounds, as a representative compound, davallialactone was also shown to suppress fat accumulation in an in vitro model system. These anti-obesity and hypolipidemic effects appear to be partly mediated by suppressing plasma and hepatic fat accumulation through the inhibition of enzymes associated with hepatic and intestinal lipid absorption and synthesis.

• Authors: Jung-Ran Noh, In-Kyoung Lee, Sun-Yung Ly, Keum-Jin Yang, Gil-Tae Gang, Yong-Hoon Kim, Jung-Hwan Hwang, Bong-Sik Yun, Chul-Ho Lee
• Journal: Journal of medicinal food (J Med Food) Vol. 14 Issue 3 Pg. 209-18 (Mar 2011) ISSN: 1557-7600 [Electronic] United States
• PMID: 21332399 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Obesity Agents
• Biological Products
• Dietary Fats
• Lactones
• RNA, Messenger
• Triglycerides
• Orlistat
• Fatty Acid Synthases

Topics

• Adipocytes (drug effects, metabolism)
• Adipose Tissue (metabolism)
• Animals
• Anti-Obesity Agents (pharmacology, therapeutic use)
• Basidiomycota (chemistry)
• Biological Products (pharmacology, therapeutic use)
• Dietary Fats (adverse effects)
• Epididymis (anatomy & histology, drug effects)
• Fatty Acid Synthases (metabolism)
• Intestinal Absorption (drug effects)
• Lactones (pharmacology)
• Lipid Metabolism (drug effects)
• Liver (drug effects, metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Obesity (chemically induced, metabolism, prevention & control)
• Orlistat
• Phytotherapy
• RNA, Messenger (metabolism)
• Random Allocation
• Triglycerides (genetics, metabolism)
• Weight Gain (drug effects)