Myocardium-derived conditioned medium improves left ventricular function in rodent acute myocardial infarction.

Abstract

BACKGROUND:

We investigated whether myocardium-derived conditioned medium (MDCM) is effective in preserving left ventricular (LV) function in a rat acute myocardial infarction (AMI) model.

METHODS:

Adult male Sprague-Dawley (SD) rats (n = 36) randomized to receive either left coronary artery ligation (AMI induction) or thoracotomy only (sham procedure) were grouped as follows (n = 6 per group): Group I, II, and III were sham-controls treated by fresh medium, normal rat MDCM, and infarct-related MDCM, respectively. Group IV, V, and VI were AMI rats treated by fresh medium, normal MDCM, and infarct-related MDCM, respectively. Either 75 μL MDCM or fresh medium was administered into infarct myocardium, followed by intravenous injection (3 mL) at postoperative 1, 12, and 24 h.

RESULTS:

In vitro studies showed higher phosphorylated MMP-2 and MMP-9, but lower α-smooth muscle actin and collagen expressions in neonatal cardiac fibroblasts treated with MDCM compared with those in the cardiac fibroblasts treated with fresh medium (all p < 0.05). Sirius-red staining showed larger collagen deposition area in LV myocardium in Group IV than in other groups (all p < 0.05). Stromal cell-derived factor-1α and CXCR4 protein expressions were higher in Group VI than in other groups (all p < 0.05). The number of von Willebrand factor- and BrdU-positive cells and small vessels in LV myocardium as well as 90-day LV ejection fraction were higher, whereas oxidative stress was lower in Group VI than in Group IV and Group V (all p < 0.05).

CONCLUSION:

MDCM therapy reduced cardiac fibrosis and oxidative stress, enhanced angiogenesis, and preserved 90-day LV function in a rat AMI model.

• Authors: Steve Leu, Ying-Hsien Kao, Cheuk-Kwan Sun, Yu-Chun Lin, Tzu-Hsien Tsai, Li-Teh Chang, Sarah Chua, Kuo-Ho Yeh, Chiung-Jen Wu, Morgan Fu, Hon-Kan Yip
• Journal: Journal of translational medicine (J Transl Med) Vol. 9 Pg. 11 (Jan 18 2011) ISSN: 1479-5876 [Electronic] England
• PMID: 21244680 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Actins
• Collagen Type I
• Culture Media, Conditioned
• smooth muscle actin, rat
• Matrix Metalloproteinases

Topics

• Actins (genetics, metabolism)
• Animals
• Animals, Newborn
• Cells, Cultured
• Collagen Type I (genetics, metabolism)
• Culture Media, Conditioned (metabolism, pharmacology)
• Fibroblasts (drug effects, metabolism, physiology)
• Gene Expression Regulation (drug effects)
• Male
• Matrix Metalloproteinases (genetics, metabolism)
• Models, Biological
• Myocardial Infarction (pathology, physiopathology)
• Myocardium (metabolism)
• Rats
• Rats, Sprague-Dawley
• Ventricular Function, Left (drug effects, physiology)