Abstract |
It remains unclear whether antibody-dependent-enhancement (ADE) of dengue infection merely augments viral attachment and entry through Fcγ receptors or immune complex binding to Fcγ receptors triggers an intrinsic signaling cascade that changes the viral permissiveness of the cell. Using human dengue- immune sera and novel human monoclonal antibodies against dengue in combination with virologic and immunologic techniques, we found that ADE infection increased the proportion of infected primary human monocytes modestly from 0.2% ± 0.1% (no Ab) to 1.7% ± 1.6% (with Ab) but the total virus output markedly from 2 ± 2 (× 10(3)) FFU to 120 ± 153 (× 10(3))FFU. However, this increased virus production was not associated with a reduced secretion of type I interferon or an elevated secretion of anti-inflammatory cytokine, IL-10. These results demonstrate that the regulation of virus production in ADE infection of primary human monocytes is more complex than previously appreciated.
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Authors | Zhihua Kou, Joanne Y H Lim, Martina Beltramello, Matthew Quinn, Huiyuan Chen, Shengyong Liu, Shengyo-ng Liu, Luis Martinez-Sobrido, Luis Martnez-Sobrido, Michael S Diamond, Jacob J Schlesinger, Aravinda de Silva, Federica Sallusto, Xia Jin |
Journal | Virology
(Virology)
Vol. 410
Issue 1
Pg. 240-7
(Feb 05 2011)
ISSN: 1096-0341 [Electronic] United States |
PMID | 21131015
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier Inc. All rights reserved. |
Chemical References |
- Antibodies, Viral
- Interferon Type I
- Interleukins
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Topics |
- Animals
- Antibodies, Viral
(physiology)
- Cells, Cultured
- Chlorocebus aethiops
- Dengue Virus
(immunology, physiology)
- Gene Expression Regulation
(physiology)
- Humans
- Interferon Type I
(genetics, metabolism)
- Interleukins
(genetics, metabolism)
- Monocytes
(physiology, virology)
- Vero Cells
- Virus Attachment
- Virus Internalization
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