Tumour-derived microvesicles (TMV) mimic the effect of tumour cells on monocyte subpopulations.

Abstract

BACKGROUND:

Monocytes/macrophages may be affected by tumour cells via cell-to-cell contact, soluble factors and by tumour-derived microvesicles (TMV). Previous observations indicate that TMV interact with monocytes and alter their immunophenotype and activity. This study was designed to determine interactions of TMV with subpopulations (CD14(++)CD16(-) and CD14(+)CD16(++)) of human monocytes.

METHODS:

Engulfment of TMV by subsets of monocytes was analysed by flow cytometry. Moreover cytokine release and production of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) by CD14(++)CD16(-) and CD14(+)CD16(++) cells after TMV stimulation was determined.

RESULTS:

It was found that TMV are engulfed more efficiently by CD14(++)CD16(-) than CD14(+)CD16(++) cells. TMV-activated CD14(++)CD16(-) cells produce more ROI and interleukin -10 (IL-10) than CD14(++)CD16(+). CD14(+)CD16(++) cells following TMV stimulation showed an increased release of tumour necrosis factor alpha, IL-12p40 and RNI.

CONCLUSION:

TMV significantly modulate biological activity of monocyte subsets with a pattern similar to tumour cells. Therefore, TMV mimic the activating effect of tumour cells on monocytes as assessed by release of cytokines, ROI and RNI.

• Authors: Monika Baj-Krzyworzeka, Jaroslaw Baran, Kazimierz Weglarczyk, Rafal Szatanek, Anna Szaflarska, Maciej Siedlar, Marek Zembala
• Journal: Anticancer research (Anticancer Res) Vol. 30 Issue 9 Pg. 3515-9 (Sep 2010) ISSN: 1791-7530 [Electronic] Greece
• PMID: 20944131 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines

Topics

• Cell Line, Tumor
• Cell Membrane (immunology)
• Cell Separation
• Cytokines (biosynthesis)
• Flow Cytometry
• Humans
• Macrophages (immunology)
• Microvessels (immunology)
• Monocytes (cytology, immunology)
• Neoplasms (blood supply, immunology)