Abstract | AIM: Recent studies have shown that lipid metabolic pathways are required for the entry, replication and secretion of hepatitis C virus (HCV). Although little is known about the life cycle of HCV in humans, the activation of cholesterol and fatty acid biosynthesis may be critical for HCV proliferation. METHODS: RESULTS: The gene expression of the LDL receptor (LDLR) was suppressed, whereas that of SREBP1c, liver X receptor-α (LXRα), fatty acid synthase (FASN), and HMG-CoA reductase and synthase (HMGR and HMGS) was significantly increased, and SREBP2 transcription was comparable in HCV-infected liver compared with normal liver. Positive correlations were found for LDLR versus HMGR, HMGR versus SREBP1c, and LDLR versus SREBP2 in the HCV-infected and control liver. Although the LXRα-SREBP1c-FASN pathway was upregulated, proteasome activator 28γ (PA28γ) was downregulated at the transcriptional level in HCV-infected liver, and was not significantly correlated with the other genes examined. The serum LDL cholesterol level was negatively correlated with LDLR and HMGR expression. CONCLUSION: These results suggest that, in HCV-infected liver, the cholesterol load increases and cholesterol uptake is controlled, while de novo cholesterol synthesis is upregulated compared with the normal physiological state. The positive correlations in the expression levels of some cholesterol metabolism-associated genes indicate that not all of the metabolic pathways are dysregulated in HCV-infected liver.
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Authors | Tatsuya Fujino, Makoto Nakamuta, Ryoko Yada, Yoko Aoyagi, Kenichiro Yasutake, Motoyuki Kohjima, Kunitaka Fukuizumi, Tsuyoshi Yoshimoto, Naohiko Harada, Masayoshi Yada, Masaki Kato, Kazuhiro Kotoh, Akinobu Taketomi, Yoshihiko Maehara, Manabu Nakashima, Munechika Enjoji |
Journal | Hepatology research : the official journal of the Japan Society of Hepatology
(Hepatol Res)
Vol. 40
Issue 9
Pg. 923-9
(Sep 2010)
ISSN: 1872-034X [Electronic] Netherlands |
PMID | 20887597
(Publication Type: Journal Article)
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