MicroRNA microarrays on archive bone marrow core biopsies of leukemias--method validation.

Abstract

Due to availability of bone marrow core biopsies (CB) in many pathology laboratories, we evaluated the quality and the biological information of the miRNA profiling using 9 acute lymphoblastic leukemia (ALL) and 9 chronic myeloid leukemia (CML) matched CB and bone marrow aspirates (BA). Technical replicates showed reproducible results across platforms and clustered together in hierarchical clustering analysis; and matched samples showed similar biological content having common differentially expressed miRNAs against the same control samples. We showed, that CBs, which have underwent decalcification in addition to formalin-fixation, are suitable for miRNA profiling.

• Authors: Ioana Borze, Mohamed Guled, Suad Musse, Anna Raunio, Erkki Elonen, Ulla Saarinen-Pihkala, Marja-Liisa Karjalainen-Lindsberg, Leo Lahti, Sakari Knuutila
• Journal: Leukemia research (Leuk Res) Vol. 35 Issue 2 Pg. 188-95 (Feb 2011) ISSN: 1873-5835 [Electronic] England
• PMID: 20828815 (Publication Type: Journal Article, Validation Study)
• Copyright: Copyright © 2010 Elsevier Ltd. All rights reserved.

Chemical References

• MicroRNAs
• Formaldehyde

Topics

• Biopsy
• Bone Marrow
• Formaldehyde
• Gene Expression Profiling (methods)
• Humans
• Leukemia (genetics)
• MicroRNAs (isolation & purification)
• Oligonucleotide Array Sequence Analysis (methods)
• Tissue Fixation