Effects of a high-protein diet on host resistance to Paracoccidioides brasiliensis in mice.

Abstract

We investigated the effects of high protein intake on host resistance to Paracoccidioides brasiliensis. Two-d fasted mice were infected with P. brasiliensis and refed on diets with three different levels (54%, 20%, and 5%) of casein. The mice refed the 54% casein diet showed reduced antifungal activity in the spleen and liver as compared with the mice refed the 5% or the 20% casein diet. After infection, increases in spleen and liver mRNA levels of myeloperoxidase, cathepsin-G, and elastase-2 were more profound in the mice refed the 54% casein diet as compared with the mice refed the 5% or the 20% casein diet. Infected mice refed the 54% casein diet exhibited greater interferon (IFN)-gamma production in the spleen and liver and higher levels of thiobarbituric acid reactive substances (TBARSs) in the liver as compared with those refed the 5% casein diet. These results indicate that high protein intake impairs host resistance to P. brasiliensis.

• Authors: Motoko Oarada, Miki Igarashi, Tsuyoshi Tsuzuki, Katsuhiko Kamei, Katsuya Hirasaka, Takeshi Nikawa, Teruo Miyazawa, Kiyotaka Nakagawa, Tohru Gonoi
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 74 Issue 3 Pg. 620-6 ( 2010) ISSN: 1347-6947 [Electronic] England
• PMID: 20208341 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Caseins
• Dietary Proteins
• Thiobarbituric Acid Reactive Substances
• Interferon-gamma
• Peroxidase
• Serine Endopeptidases
• Cathepsin G
• pancreatic elastase II

Topics

• Animals
• Caseins (immunology)
• Cathepsin G (analysis)
• Dietary Proteins (administration & dosage)
• Female
• Host-Pathogen Interactions (immunology)
• Interferon-gamma (metabolism)
• Liver (enzymology, immunology)
• Mice
• Mice, Inbred BALB C
• Paracoccidioides (immunology)
• Paracoccidioidomycosis (immunology)
• Peroxidase (analysis)
• Serine Endopeptidases (analysis)
• Spleen (enzymology, immunology)
• Thiobarbituric Acid Reactive Substances (metabolism)