[Effects of silencing chloride intracellular channel 1 gene expression on the proliferation and invasion of mouse hepatocellular carcinoma cell lines].

Abstract

OBJECTIVE:

To study the effects of silencing CLIC1 gene expression on the proliferation and invasion of Hca-F cells.

METHODS:

The mouse CLIC1 cDNA sequence was retrieved from NCBI. Three shRNA sequences were designed and cloned into pGPU6/GFP/Neo plasmids. The plasmids were transfected into Hca-F cells with Lipofectamine 2000. Cell Counting-8 (CCK-8) kit and transwell chamber were used to study the effects of CLIC1 on the proliferation and invasion of Hca-F cells.

RESULTS:

The pGPU6/GFP/Neo-shRNA-3 plasmid effectively repressed the expression of CLIC1 mRNA. Inhibition of CLIC1 gene expression led to decreased cell proliferation and reduced invasion.

CONCLUSION:

CLIC1 is essential for the proliferation and invasion of Hca-F cells.

• Authors: Rong-kuan Li, Jian-wu Tang, Jun Zhang, Shao-qing Wang, Mei Wang, Bo Wang, Yu-hong Zhang
• Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (Zhonghua Gan Zang Bing Za Zhi) Vol. 18 Issue 2 Pg. 131-5 (Feb 2010) ISSN: 1007-3418 [Print] China
• PMID: 20196953 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chloride Channels
• Clic1 protein, mouse
• RNA, Messenger
• RNA, Small Interfering

Topics

• Animals
• Carcinoma, Hepatocellular (metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Chloride Channels (antagonists & inhibitors, genetics, metabolism)
• Gene Expression Regulation, Neoplastic
• Genetic Vectors
• Liver Neoplasms (metabolism, pathology)
• Mice
• Neoplasm Invasiveness
• Plasmids (genetics)
• RNA Interference
• RNA, Messenger (genetics, metabolism)
• RNA, Small Interfering (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Transfection