Antigen-specific immunoadsorption of anti-acetylcholine receptor antibodies from sera of patients with myastenia gravis.

Abstract

BACKGROUND:

The binding of anti-acetylcholine receptor antibodies (AChRAb) to the main immunogenic region (MIR) of AChR alpha-subunit in the neuromuscular junction is the major pathogenesis of myasthenia gravis (MG).

METHODS:

A synthetic peptide of 10 amino acids corresponding to the MIR of human AChR was coupled with cellulose beads to make an antigen-specific immunoadsorbent (hMIR10-CB).

RESULTS:

The hMIR10-CB could remove AChRAb in MG sera by 40.3+/-2.3%, compared to a tryptophan nonspecific adsorbent Trp-CB by only 22.4+/-1.5% as determined in ELISA, and also showed good blood compatibility for blood cells, plasma ions and plasma proteins as checked in whole blood perfusion in rabbits.

CONCLUSIONS:

The antigen-specific immunoadsorbent hMIR10-CB can serve as a potential candidate in the immunoadsorption treatment of MG.

• Authors: Changyuan Sun, Fanping Meng, Yingxin Li, Quanxin Jin, Honghua Li, Fangfang Li
• Journal: Artificial cells, blood substitutes, and immobilization biotechnology (Artif Cells Blood Substit Immobil Biotechnol) Vol. 38 Issue 2 Pg. 99-102 (Apr 2010) ISSN: 1532-4184 [Electronic] England
• PMID: 20196680 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Autoantibodies
• Immunodominant Epitopes
• Peptide Fragments
• Receptors, Cholinergic
• Tryptophan
• Cellulose

Topics

• Animals
• Autoantibodies (blood, immunology)
• Blood Component Removal
• Cellulose (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Hematologic Tests
• Humans
• Immunodominant Epitopes
• Immunosorbent Techniques
• Microspheres
• Myasthenia Gravis (blood, immunology, therapy)
• Peptide Fragments (metabolism)
• Rabbits
• Receptors, Cholinergic (immunology)
• Tryptophan (metabolism)