Serodiagnostic efficacy of Mycobacterium tuberculosis 30/32-kDa mycolyl transferase complex, ESAT-6, and CFP-10 in patients with active tuberculosis.

Abstract

Elimination of tuberculosis (TB) largely depends upon definitive rapid diagnosis and treatment. Widely used diagnostic tests do not qualify for use in a developing country due to lack of either desired accuracy or their cost. In the present study an enzyme-linked immunosorbent assay was used to evaluate the diagnostic potential of an immuno-dominant 30/32-kDa mycolyl transferase complex (Ag85 complex) and Mycobacterium tuberculosis-specific proteins (ESAT-6 and CFP-10) of the RD1 region. Higher sensitivity (84.1%) with Ag85 complex was observed compared with ESAT-6 (64.9%) and CFP-10 (66%), with almost similar specificity (Ag85: 85.2%, ESAT-6: 88.9%, CFP-10: 85.2%), whereas the individual components of Ag85 complex, i.e. Ag85A, Ag85B, and Ag85C, showed sensitivities of 44.6, 34, and 80.9% and specificities of 55.6, 74.1, and 40.7% respectively. A cocktail of Ag85 complex, ESAT-6, CFP-10, Ag85A, Ag85B, and Ag85C antigens also could not help in increasing either sensitivity (51.1%) or specificity (85.2%). Furthermore, immunoblot analysis using clinical isolates as well as a standard strain (H37Rv) of M. tuberculosis also showed strong reactivity of sera from TB patients to Ag85 complex and, to a lesser extent, also to ESAT-6. To conclude, use of Ag85 complex along with ESAT-6 and CFP-10 seems to be promising in minimizing the heterogeneous sero-responses of adult TB cases.

• Authors: Gavish Kumar, Pradeep Kumar Dagur, Prashant Kumar Singh, Hari Shankar, Virendra S Yadav, Vishwa M Katoch, Bharat Bajaj, Rajesh Gupta, Utpal Sengupta, Beenu Joshi
• Journal: Archivum immunologiae et therapiae experimentalis (Arch Immunol Ther Exp (Warsz)) Vol. 58 Issue 1 Pg. 57-65 (Feb 2010) ISSN: 1661-4917 [Electronic] Switzerland
• PMID: 20049651 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial
• Bacterial Proteins
• CFP-10 protein, Mycobacterium tuberculosis
• ESAT-6 protein, Mycobacterium tuberculosis
• Acyltransferases
• antigen 85B, Mycobacterium tuberculosis

Topics

• Acyltransferases (analysis, immunology)
• Adolescent
• Adult
• Aged
• Antigens, Bacterial (analysis, immunology)
• Bacterial Proteins (analysis, immunology)
• Enzyme-Linked Immunosorbent Assay
• Feasibility Studies
• Female
• Humans
• Male
• Middle Aged
• Mycobacterium tuberculosis (immunology, metabolism, pathogenicity)
• Sensitivity and Specificity
• Tuberculosis (diagnosis)