Antiangiogenic properties of a nutrient mixture in a model of hemangioma.

Abstract

UNLABELLED:

The pathogenesis of hemangiomas is still largely unknown and the current therapy, such as systemic corticosteroid, vincristine, and interferon-alpha, is toxic and remains unsatisfactory. A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract has shown significant anti-angiogenic and anti-tumor effect against a number of cancer cell lines.

AIM:

Using a mouse hemangioendothelioma model, we investigated the efficacy of NM. We also tested the effect of NM in vitro, evaluating cell viability, MMP secretion, invasion, morphology and apoptosis.

METHODS:

Athymic nude mice, 5-6 weeks old, were inoculated with 3 x10(6) EOMA cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B - a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. We also tested the effect of NM in vitro.

RESULTS:

NM inhibited the growth of tumors by 50%. In vitro, NM exhibited dose response cytotoxicity with 10%, 30% and 55% at 10, 100 and 1000 microg/ml. Invasion through Matrigel was inhibited at 50, 100 and 500 microg/ml by 25%, 30% and 100% respectively. NM induced dose-dependent apoptosis of EOMA cells.

CONCLUSIONS:

These results suggest that NM may have therapeutic potential in treating infantile hemangioendotheliomas and, perhaps, other cutaneous vascular tumors.

• Authors: M W Roomi, T Kalinovsky, A Niedzwiecki, M Rath
• Journal: Experimental oncology (Exp Oncol) Vol. 31 Issue 4 Pg. 214-9 (Dec 2009) ISSN: 2312-8852 [Electronic] Ukraine
• PMID: 20010532 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiogenesis Inhibitors
• Plant Extracts
• Proline
• Lysine
• Ascorbic Acid

Topics

• Angiogenesis Inhibitors (pharmacology)
• Animals
• Apoptosis (drug effects)
• Ascorbic Acid (pharmacology)
• Camellia sinensis (chemistry)
• Cell Line, Tumor
• Hemangioendothelioma (drug therapy, pathology)
• Lysine (pharmacology)
• Male
• Mice
• Mice, Nude
• Neoplasms, Experimental (drug therapy, pathology)
• Phytotherapy (methods)
• Plant Extracts (pharmacology)
• Proline (pharmacology)