Abstract | OBJECTIVES: METHODS: RESULTS: The frequency of positive WB findings in SLE (18/73, 25%) was higher than in other diseases. ELISA kits A and B for anti-P antibodies showed 21% and 43% sensitivity, and 93% and 88% specificity, respectively, for diagnosing SLE, based on the manufacturer's cut-off values. Receiver operating characteristic (ROC) curve analysis, based on positive WB findings, determined a new cut-off threshold but revealed that both ELISA kits still had good diagnostic characteristics. In IIF assays on anti-P antibody positive sera, typical anti-P antibody cytoplasmic staining patterns (n=8) were seen less frequently than other staining patterns (n=17). CONCLUSIONS: Routine screening for anti-P antibodies by IIF has low sensitivity. ELISAs using cut-off values established by individual facilities are suitable for detecting anti-P antibodies and provide a tool with good diagnostic characteristics, on a parity with WB.
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Authors | Y Muro, K Sugiura, Y Morita, Y Tomita |
Journal | Scandinavian journal of rheumatology
(Scand J Rheumatol)
2009 Nov-Dec
Vol. 38
Issue 6
Pg. 460-3
ISSN: 1502-7732 [Electronic] England |
PMID | 19922022
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Autoantibodies
- Phosphoproteins
- Ribosomal Proteins
- phosphoprotein P2, ribosomal
- ribosomal phosphoprotein P1
- ribosomal protein P0
- Protein Serine-Threonine Kinases
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Topics |
- Adult
- Autoantibodies
(analysis)
- Blotting, Western
(methods)
- Connective Tissue Diseases
(diagnosis, ethnology, immunology)
- Diagnosis, Differential
- Enzyme-Linked Immunosorbent Assay
(methods)
- Female
- Fluorescent Antibody Technique, Indirect
(methods)
- Follow-Up Studies
- Humans
- Japan
(epidemiology)
- Male
- Middle Aged
- Phosphoproteins
(immunology)
- Prevalence
- Protein Serine-Threonine Kinases
- Ribosomal Proteins
(immunology)
- Sensitivity and Specificity
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