Screening for genetic abnormalities involved in ovarian carcinogenesis using retroviral expression libraries.

Abstract

The purpose of this study was to screen for genes involved in ovarian carcinogenesis in an attempt to develop an effective molecular-targeted therapy for ovarian cancer. We constructed retroviral expression libraries for the human ovarian cancer cell lines SHIN-3 and TYK-CPr, and performed a focus formation assay with 3T3 cells. As a result, proteasome subunit beta-type 2 (PSMB2), ubiquitin-specific protease 14 (USP14), and keratin 8 (KRT8) were identified from SHIN-3, and polymerase II RNA subunit (POLR2E), chaperonin containing T-complex polypeptide 1 subunit 4 (CCT4), glia maturation factor beta (GMFB), and neuroblastoma ras viral oncogene homolog (NRAS) from TYK-CPr. NRAS gene analysis revealed a CAA --> AAA substitution at codon 61, resulting in a Glu --> Lys change at position 61. When the mutant NRAS was introduced into fibroblasts for its expression, many transformed foci were generated, confirming the transforming ability of the mutant NRAS.

• Authors: Tomoaki Wada, Yoshihiro Yamashita, Yasushi Saga, Kayoko Takahashi, Koji Koinuma, Young Lim Choi, Ruri Kaneda, Shin-Ichiro Fujiwara, Manabu Soda, Hideki Watanabe, Kentaro Kurashina, Hisashi Hatanaka, Munehiro Enomoto, Shuji Takada, Hiroyuki Mano, Mitsuaki Suzuki
• Journal: International journal of oncology (Int J Oncol) Vol. 35 Issue 5 Pg. 973-6 (Nov 2009) ISSN: 1791-2423 [Electronic] Greece
• PMID: 19787249 (Publication Type: Journal Article)

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Cell Line, Tumor
• Female
• Gene Library
• Genes, ras (genetics)
• Genetic Testing (methods)
• Humans
• Mice
• Molecular Sequence Data
• Ovarian Neoplasms (genetics)
• Retroviridae (genetics)
• Reverse Transcriptase Polymerase Chain Reaction