Glaucoma, one of the leading causes of irreversible
blindness, is characterized by progressive degeneration of retinal ganglion cells (RGCs) and optic nerves. Although
glaucoma is often associated with elevated intraocular pressure, recent studies have shown a relatively high prevalence of
normal tension glaucoma (NTG) in
glaucoma patient populations. In the mammalian retina,
glutamate/aspartate transporter (GLAST) is localized to Müller glial cells, whereas
excitatory amino acid carrier 1 (EAAC1) is expressed in neural cells, including RGCs. Since the loss of GLAST or EAAC1 leads to
retinal degeneration similar to that seen in NTG, we examined the effects of
interleukin-1 (IL-1) on RGC death in GLAST- and EAAC1-deficient mice.
IL-1 promoted increased
glutamate uptake in Müller cells by suppressing intracellular Na(+) accumulation, which is necessary to counteract Na(+)-
glutamate cotransport. The observed trends for the
glutamate uptake increase in the wild-type (WT), GLAST- and EAAC1-deficient mice were similar; however, the baseline
glutamate uptake and intracellular Na(+) concentration in the GLAST-deficient mice were significantly lower than those in the wild-type mice. Consistently, pretreatment with
IL-1 exhibited no beneficial effects on
glutamate-induced RGC degeneration in the GLAST-deficient mice. In contrast,
IL-1 significantly increased
glutamate uptake by Müller cells and the number of surviving RGCs in the wild-type and EAAC1-deficient mice. Our findings suggest that the use of
IL-1 for enhancing the function of
glutamate transporters may be useful for neuroprotection in
retinal degenerative disorders including NTG.