[Influences of the phosphorylation of p38 mitogen activated protein kinase on gene expression of tumor necrosis factor-alpha in multiple organ dysfunction syndrome in pigs].

Abstract

OBJECTIVE:

To investigate that the phosphorylation of the p38 mitogen activated protein kinase (p38MAPK) influences gene expression of tumor necrosis factor-alpha (TNF-alpha) in multiple organ dysfunction syndrome (MODS) in pigs.

METHODS:

Thirty pigs were divided into MODS group and control group, and an animal model of MODS of "two-hit" injury, including hemorrhagic shock and endotoxemia, was reproduced. The content of p38MAPK's phosphorylation was assessed with Western blotting. TNF-alpha mRNA in peripheral blood monocytes was assayed with real time-polymerase chain reaction (RT-PCR). TNF-alpha was monitored in the peripheral blood plasma with enzyme linked immunosorbent assay (ELISA).

RESULTS:

Phosphorylation of p38MAPK was obviously increased in extent, which enhanced gene expression of TNF-alpha and then secretion of TNF-alpha by the peripheral blood mononuclear cell in MODS, and the differences were statistically significant compared with that of control group (P<0.05 or P<0.01).

CONCLUSION:

p38MAPK's phosphorylation is important in pathogenesis of MODS, and phosphorylation of p38MAPK can enhance TNF-alpha mRNA transcription and secretion of TNF-alpha from peripheral blood mononuclear cells, which is the mechanism of increased TNF-alpha in MODS.

• Authors: An-rong Mao, He Huang, Guo-en Fang, Li-ye Ma, Jian-wei Bi, Tian-hang Luo, Wen-zheng Fu, Ji-de Hua
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 21 Issue 9 Pg. 518-20 (Sep 2009) ISSN: 1003-0603 [Print] China
• PMID: 19751557 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• Tumor Necrosis Factor-alpha
• p38 Mitogen-Activated Protein Kinases

Topics

• Animals
• Disease Models, Animal
• Gene Expression Regulation
• Male
• Multiple Organ Failure (metabolism)
• Phosphorylation
• RNA, Messenger (genetics)
• Random Allocation
• Swine
• Tumor Necrosis Factor-alpha (genetics, metabolism)
• p38 Mitogen-Activated Protein Kinases (metabolism)