Diacerein decreases TNF-alpha and IL-1beta levels in peritoneal fluid and prevents Baker's yeast-induced fever in young rats.

Abstract

OBJECTIVE:

To investigate the effect of diacerein, an anti-inflammatory drug, on body temperature and protocols of fever induction in male Wistar rats.

METHODS:

The effect of diacerein (5.0 mg/kg, s.c.) on rectal temperature (T (R)) changes induced by Baker's yeast (0.135 g/kg, i.p.) and PGE(2) (10 ng/animal, i.t.) was evaluated. T (R) changes were recorded over time. The leukocyte count and TNF-alpha and IL-1beta content were evaluated in the peritoneal fluid by means of optical microscopy and enzyme immunoassay (ELISA kits), respectively.

RESULTS:

The administration of diacerein to febrile animals attenuated Baker's yeast-induced fever but did not alter prostaglandin E(2)-induced fever. Diacerein prevented the development of Baker's yeast-induced fever and significantly attenuated the increase in peritoneal leukocytes and decreased IL-1beta and TNF-alpha levels in peritoneal fluid.

CONCLUSIONS:

These data suggest that diacerein partially protects against Baker's yeast-induced fever and peritoneal leukocyte migration, and indicate that this effect appears to be due to inhibition of release of cytokines (such as TNF-alpha and IL-1beta).

• Authors: Juliana Saibt Martins Pasin, Ana Paula Oliveira Ferreira, André Luis Lopes Saraiva, Viviane Ratzlaff, Rosália Andrighetto, Jorgete Tomazetti, Daiana Silva Avila, Sydney Hartz Alves, Maribel Antonello Rubin, Juliano Ferreira, Adair Roberto Soares Santos, Carlos Fernando Mello
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 59 Issue 3 Pg. 189-96 (Mar 2010) ISSN: 1420-908X [Electronic] Switzerland
• PMID: 19730987 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anthraquinones
• Anti-Inflammatory Agents
• Interleukin-1beta
• Tumor Necrosis Factor-alpha
• diacerein
• Dinoprostone

Topics

• Animals
• Anthraquinones (pharmacology)
• Anti-Inflammatory Agents (pharmacology)
• Ascitic Fluid (metabolism)
• Body Temperature (drug effects)
• Cell Movement (drug effects)
• Dinoprostone (adverse effects)
• Disease Models, Animal
• Fever (chemically induced, prevention & control)
• Interleukin-1beta (metabolism)
• Leukocytes (cytology, drug effects)
• Male
• Rats
• Rats, Wistar
• Saccharomyces cerevisiae
• Tumor Necrosis Factor-alpha (metabolism)