Intranasal administration of CpG DNA lipoplex prevents pulmonary metastasis in mice.

Abstract

Synthetic oligodeoxynucleotides containing CpG motifs (CpG DNA) can activate immunocompetent cells which offer the potential advantage of antitumor activity. In this study, we used cationic liposomes to complex with CpG DNA (CpG DNA lipoplex) to prevent pulmonary metastasis following intranasal administration in mice. Intranasal administration of CpG DNA lipoplex prior to challenge with both colon26/Luc and B16F10 cells significantly prevented the proliferation of tumor cells, and the survival time of the mice receiving CpG DNA lipoplex was prolonged. After intranasal administration, [(32)P] CpG DNA lipoplex mainly distributed in nose and lung and induced higher IFN-gamma production in the lung. These results suggest that intranasal administration of CpG DNA lipoplex has a significant effect on preventing pulmonary metastasis in mice.

• Authors: Shuwen Zhou, Shigeru Kawakami, Fumiyoshi Yamashita, Mitsuru Hashida
• Journal: Cancer letters (Cancer Lett) Vol. 287 Issue 1 Pg. 75-81 (Jan 01 2010) ISSN: 1872-7980 [Electronic] Ireland
• PMID: 19660857 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• CPG-oligonucleotide
• Cytokines
• Liposomes
• Oligodeoxyribonucleotides
• Aspartate Aminotransferases
• Alanine Transaminase

Topics

• Adjuvants, Immunologic (administration & dosage)
• Administration, Intranasal
• Alanine Transaminase (blood)
• Animals
• Aspartate Aminotransferases (blood)
• Cell Line, Tumor
• Colonic Neoplasms (drug therapy, pathology)
• Cytokines (biosynthesis)
• Liposomes (administration & dosage)
• Lung Neoplasms (prevention & control, secondary)
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Oligodeoxyribonucleotides (administration & dosage, pharmacokinetics)