[Screening and identification analysis of serum protein biomarkers in nephroblastoma].

Abstract

OBJECTIVE:

To screen and characterize the serum protein biomarkers in nephroblastoma so as to establish the proteins as the specific serum biomarkers for diagnosis and prognosis monitoring.

METHODS:

The differential protein peaks were located by detecting serum samples of preoperative and postoperative patients and normal children using the SELDI-TOF MS technology. After purification, the differential proteins were further analyzed by LC-MS/MS and the protein sequences searched in database.

RESULTS:

Two peaks with m/z of 6455.5 and 6984.4 were selected as potential biomarkers. They were weakly expressed in nephroblastoma (intensity: 1029 +/- 364, 297 +/- 126) but highly expressed in normal individuals (2108 +/- 837, 753 +/- 226); another peak with m/z of 9190.8 was weakly expressed in preoperative sera (283 +/- 154) but highly expressed in serum samples of postoperative patients and normal children (5974 +/- 657, 6231 +/- 519). The protein at 6455.5 and 9190.8 were identified as apolipoprotein C-III and haptoglobin respectively.

CONCLUSION:

The detection of differentially expressed apolipoprotein C-III and haptoglobin may have potential utilities for serum diagnosis, malignancy classification and prognosis monitoring of nephroblastoma and is worthy of further studies and applications.

• Authors: Jia-Xiang Wang, Shao-Bo Yang, Qiu-Liang Liu, Rui Dong, Yu-Xia Fan, Qian Zhang, Fu-Quan Yang, Peng Wu, Jie-Kai Yu, Shu Zheng
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 89 Issue 18 Pg. 1259-63 (May 12 2009) ISSN: 0376-2491 [Print] China
• PMID: 19595180 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apolipoprotein C-III
• Biomarkers, Tumor
• Blood Proteins
• Haptoglobins
• Neoplasm Proteins

Topics

• Apolipoprotein C-III (blood)
• Biomarkers, Tumor (blood)
• Blood Proteins (analysis)
• Case-Control Studies
• Child
• Child, Preschool
• Female
• Haptoglobins (analysis)
• Humans
• Infant
• Infant, Newborn
• Male
• Neoplasm Proteins (blood)
• Neoplasm Staging
• Proteomics
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Wilms Tumor (blood, pathology)