Ginsenoside Rg1 protects rat cardiomyocyte from hypoxia/reoxygenation oxidative injury via antioxidant and intracellular calcium homeostasis.

Abstract

Ginsenoside Rg1 is a major active ingredient of Panax notoginseng radix which has demonstrated a number of pharmacological actions including a cardioprotective effect in vivo. This study investigated the protective effect and mechanism of ginsenoside Rg1 in cardiomyocytes hypoxia/reoxygenation (H/R) model. Pretreatment with ginsenoside Rg1 (60-120 microM) reduced lactate dehydrogenase release and increased cell viability in a dose-dependent manner. Fluorescence analysis demonstrated ginsenoside Rg1 reduced intracellular ROS and suppressed the intracellular [Ca(2+)] level. Cell lysate detected an increase of T-SOD, CAT, and GSH levels. The myocardial protection of ginsenoside Rg1 during H/R is partially due to its antioxidative effect and intracellular calcium homeostasis.

• Authors: Dan Zhu, Lei Wu, Chun-Rong Li, Xiao-Wei Wang, Yong-Jie Ma, Zhi-Yin Zhong, Hai-Bao Zhao, Jian Cui, Si-Fan Xun, Xiu-Lan Huang, Zhe Zhou, Sheng-Qi Wang
• Journal: Journal of cellular biochemistry (J Cell Biochem) Vol. 108 Issue 1 Pg. 117-24 (Sep 01 2009) ISSN: 1097-4644 [Electronic] United States
• PMID: 19530220 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: (c) 2009 Wiley-Liss, Inc.

Chemical References

• Antioxidants
• Ginsenosides
• Reactive Oxygen Species
• ginsenoside Rg1
• Oxygen
• Calcium

Topics

• Animals
• Antioxidants (metabolism)
• Calcium (metabolism)
• Cell Hypoxia
• Cell Survival
• Female
• Ginsenosides (pharmacology)
• Homeostasis
• Male
• Myocytes, Cardiac (drug effects, metabolism)
• Oxidation-Reduction
• Oxidative Stress
• Oxygen (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reactive Oxygen Species (metabolism)