Abstract | AIMS: MAIN METHODS: KEY FINDINGS:
Silymarin treatment restored the values for collagen content and ALT and ALP activities when compared to the values with spontaneous resolution following discontinuation of CCl4. CCl4 treatment highly increased eNOS expression and NOS activity in livers, but the phosphorylation was markedly decreased. Silymarin decreased significantly eNOS expression and activity. Expression and/or phosphorylation of enzymes activating eNOS were unchanged (Akt and AMPK) or decreased (PKA) by silymarin. Especially, the expression of caveolin-1, an inhibitor of eNOS was unchanged by CCl4, but its phosphorylation was significantly increased. However, silymarin markedly increased caveolin-1 expression but decreased its phosphorylation to expression. SIGNIFICANCE: These results suggest that chronic silymarin treatment can improve cirrhosis-induced liver enzyme activities and fibrosis, but may aggravate the hemodynamic eNOS activity, particularly by decreasing eNOS expression and increasing caveolin-1 expression.
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Authors | Yong Kyun Cho, Jung Won Yun, Jung Ho Park, Hong Joo Kim, Dong Il Park, Chong Il Sohn, Woo Kyu Jeon, Byung Ik Kim, Wook Jin, Yong-Hyun Kwon, Mi-Kyung Shin, Tae Moo Yoo, Ju-Hee Kang, Chang-Shin Park |
Journal | Life sciences
(Life Sci)
Vol. 85
Issue 7-8
Pg. 281-90
(Aug 12 2009)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 19527736
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Protective Agents
- Silymarin
- Collagen
- Carbon Tetrachloride
- Nitric Oxide Synthase Type III
- Nos3 protein, rat
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Topics |
- Animals
- Blotting, Western
- Carbon Tetrachloride
- Collagen
(metabolism)
- Gene Expression
(drug effects)
- Liver Cirrhosis, Experimental
(chemically induced, enzymology, prevention & control)
- Liver Function Tests
- Male
- Nitric Oxide Synthase Type III
(genetics, metabolism)
- Protective Agents
(administration & dosage, therapeutic use)
- Rats
- Rats, Sprague-Dawley
- Silymarin
(administration & dosage, therapeutic use)
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