Abstract | BACKGROUND:
Phospholipids are essential for the normal function of the intestinal mucus barrier. The objective of this study was to systematically investigate phospholipids in the intestinal mucus of humans suffering from inflammatory bowel diseases, where a barrier defect is strongly supposed to be pathogenetic. METHODS: RESULTS: Human and rodent mucus contained very similar phospholipid species. In the ileal and colonic mucus from patients suffering from UC, the concentration of PC was highly significantly lower (607 +/- 147 pmol/100 microg protein and 745 +/- 148 pmol/100 microg protein) compared to that of patients with CD (3223 +/- 1519 pmol/100 microg protein and 2450 +/- 431 pmol/100 microg protein) and to controls (3870 +/- 760 pmol/100 microg protein and 2790 +/- 354 pmol/100 microg protein); overall, P = 0.0002 for ileal specimens and P < 0.0001 for colonic specimens. Independent of disease activity, patients suffering from UC showed an increased saturation grade of PC fatty acid residues and a higher LPC-to-PC ratio. CONCLUSIONS: The intestinal mucus barrier of patients with UC is significantly altered concerning its phospholipid concentration and species composition. These alterations may be very important for the pathogenesis of this disease and underline new therapeutic strategies.
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Authors | Annika Braun, Irina Treede, Daniel Gotthardt, Anke Tietje, Alexandra Zahn, Rebecca Ruhwald, Ulrike Schoenfeld, Thilo Welsch, Peter Kienle, Gerhard Erben, Wolf-Dieter Lehmann, Joachim Fuellekrug, Wolfgang Stremmel, Robert Ehehalt |
Journal | Inflammatory bowel diseases
(Inflamm Bowel Dis)
Vol. 15
Issue 11
Pg. 1705-20
(Nov 2009)
ISSN: 1536-4844 [Electronic] England |
PMID | 19504612
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adrenal Cortex Hormones
- Lysophosphatidylcholines
- Phosphatidylcholines
- Phospholipids
- Sphingomyelins
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Topics |
- Adrenal Cortex Hormones
(therapeutic use)
- Animals
- Colitis, Ulcerative
(drug therapy, etiology, metabolism, pathology)
- Colon
(metabolism, pathology)
- Colonoscopy
- Female
- Humans
- Ileum
(metabolism, pathology)
- Intestinal Mucosa
(metabolism, pathology)
- Lysophosphatidylcholines
(metabolism)
- Mice
- Mice, Inbred C57BL
- Mice, Inbred CBA
- Middle Aged
- Mucus
(metabolism)
- Phosphatidylcholines
(metabolism)
- Phospholipids
(metabolism)
- Spectrometry, Mass, Electrospray Ionization
- Sphingomyelins
(metabolism)
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