Constitutive neuronal expression of the immune regulator, programmed death 1 (PD-1), identified during experimental autoimmune uveitis.

Abstract

PURPOSE:

Programmed death-1 (PD-1) ligation downregulates active lymphocyte responses. The authors tested whether PD-1 or its ligands are expressed in the posterior segment during active intraocular inflammation.

METHODS:

Experimental autoimmune uveitis (EAU) was induced using interphotoreceptor retinoid-binding protein (IRBP 161-180). Ocular inflammation was evaluated by histology and expression of PD-1 ligand tested by immunohistochemistry. PD-1 expression was evaluated by immunohistochemistry, RT-PCR, and Western immunoblotting.

RESULTS:

Using immunohistochemistry, PD-1, but not its ligands, was constitutively expressed in retinal neurons of naive mouse retina. Both PD-1 and its ligands were observed, as expected, in sites of active inflammation.

CONCLUSIONS:

PD-1 and its ligands were expressed in sites of active inflammation, in accordance with many other models of inflammatory disease. Surprisingly, PD-1, not previously described outside the immune system, was constitutively expressed in retinal neurons, raising the possibility that PD-1 signaling may be important for neuronal function in the absence of an inflammatory insult.

• Authors: Ling Chen, Vicky Pai, Ralph Levinson, Arlene H Sharpe, Gordon J Freeman, Jonathan Braun, Lynn K Gordon
• Journal: Ocular immunology and inflammation (Ocul Immunol Inflamm) 2009 Jan-Feb Vol. 17 Issue 1 Pg. 47-55 ISSN: 1744-5078 [Electronic] England
• PMID: 19294574 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Surface
• Apoptosis Regulatory Proteins
• B7-1 Antigen
• B7-H1 Antigen
• Cd274 protein, mouse
• Eye Proteins
• Membrane Glycoproteins
• Pdcd1 protein, mouse
• Pdcd1lg2 protein, mouse
• Peptide Fragments
• Peptides
• Programmed Cell Death 1 Ligand 2 Protein
• Programmed Cell Death 1 Receptor
• RNA, Messenger
• Retinol-Binding Proteins
• interstitial retinol-binding protein

Topics

• Animals
• Antigens, Surface (genetics, metabolism)
• Apoptosis Regulatory Proteins (genetics, metabolism)
• Autoimmune Diseases (genetics, metabolism, pathology)
• B7-1 Antigen (genetics, metabolism)
• B7-H1 Antigen
• Blotting, Western
• Brain (metabolism)
• Disease Models, Animal
• Eye Proteins
• Immunoenzyme Techniques
• Male
• Membrane Glycoproteins (genetics, metabolism)
• Mice
• Peptide Fragments
• Peptides (genetics, metabolism)
• Programmed Cell Death 1 Ligand 2 Protein
• Programmed Cell Death 1 Receptor
• RNA, Messenger (metabolism)
• Retinol-Binding Proteins
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Analysis, DNA
• Uveitis, Posterior (genetics, metabolism, pathology)