[Experimental study on therapeutic efficacy of tetrandrine on lung injury induced by acute of paraquat poisoning].

Abstract

OBJECTIVE:

To investigate the antagonistic efficacy of tetrandrine (TET) on lung injury induced by acute paraquat poisoning.

METHODS:

Male Wistar rats were randomly divided into three groups (control group, non-treatment group and treatment group). The tetrandrine of 30 mg/kg was given by gastric lavage six hours after 32 rats were intraperitoneally injected with paraquat 15 mg/kg (treatment group). Then the same dose of tetrandrine was given once a day. Normal saline of the same volume was given by gastric lavage in another 32 rats intraperitoneally injected with paraquat 15 mg/kg (non-treatment group). Seven rats were intraperitoneally injected by normal saline as the control group. Levels of maleic dialdehyde (MDA), activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in plasma and the lung homogenate of three groups were determined at 3 d, 7 d, 14 d and 21 d after exposure to paraquat. Histological changes of the lungs were observed.

RESULTS:

The levels of MDA at 3 d both in plasma [(3.65 +/- 0.44) nmol/ml] and the lung homogenate [(9.54 +/- 0.92) nmol/mg pro] of the non-treatment group significantly increased compared with the control group (P < 0.01), the activities of GSH-Px and SOD in plasma at 3 d were significantly less than the control group (P < 0.05 or P < 0.01), the activities of GSH-Px (3 d, 7 d) and SOD (7 d, 14 d) in the lung homogenate were significantly less than the control group (P < 0.05). There were no significant difference in the levels of MDA both in plasma and the lung homogenate between the treatment group and the non-treatment group (P > 0.05). The SOD activities of treatment group on the third day was significantly increased compared with the non-treatment group (P < 0.01 or P < 0.05). Although the activities of GSH-Px in plasma and the lung homogenate of the treatment group on the third day were increased, there was no significantly difference compared with the non-treatment group (P > 0.05). The integral score of pulmonary fibrosis in the treatment group were significantly lower than in the non-treatment group (P < 0.01).

CONCLUSION:

TET has antagonistic effect against acute toxicity of paraquat through significant reduction of pulmonary fibrosis.

• Authors: Qiu-Hong Zhu, Jin-Xiang Huang, Cong-Shen Meng, Ming-Liang Wang, Wei Chen, Xing Zhang
• Journal: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases (Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi) Vol. 26 Issue 10 Pg. 583-7 (Oct 2008) ISSN: 1001-9391 [Print] China
• PMID: 19272249 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzylisoquinolines
• tetrandrine
• Malondialdehyde
• Glutathione Peroxidase
• Superoxide Dismutase
• Paraquat

Topics

• Acute Disease
• Animals
• Benzylisoquinolines (therapeutic use)
• Disease Models, Animal
• Glutathione Peroxidase (metabolism)
• Lung (drug effects, metabolism, pathology)
• Lung Injury (chemically induced, drug therapy, metabolism, pathology)
• Male
• Malondialdehyde (metabolism)
• Paraquat (poisoning)
• Rats
• Rats, Wistar
• Superoxide Dismutase (metabolism)