Plasma-based detection of clonality in lymphoid malignancies.

Abstract

OBJECTIVES:

Plasma has been found to be enriched with tumor-specific DNA, RNA, and protein in patients with hematologic disease. We assessed the utility of plasma as a DNA source for detection of genetic abnormalities in patients with suspected B- or T-cell lymphoproliferative disorders.

METHODS:

DNA was extracted from paired peripheral blood (PB) cells and plasma for polymerase chain reaction (PCR)-based detection of immunoglobulin heavy chain (IgH) and T-cell receptor gamma chain (TCR-gamma) rearrangements, and B-cell leukemia/lymphoma (BCL)-1/IgH and BCL-2/IgH translocations.

RESULTS:

Concordance between plasma and PB cell analysis was 100% for IgH (n = 57), TCR-gamma (n = 57), and BCL-1/IgH (n = 37) rearrangements, and 94% (60/64) for BCL-2/IgH; four of 11 plasma samples positive for BCL-2/IgH tested negative in paired cells. No plasma or PB cell samples from 195 healthy donors showed genetic abnormalities.

CONCLUSIONS:

These findings indicate that plasma is a reliable sample type for detection of abnormalities associated with B- and T-cell lymphoproliferative disorders, providing sensitivity equal to or greater than that of PB cells.

• Authors: Chen-Hsiung Yeh, Richard Tseng, Maher Albitar
• Journal: European journal of haematology (Eur J Haematol) Vol. 82 Issue 6 Pg. 450-3 (Jun 2009) ISSN: 1600-0609 [Electronic] England
• PMID: 19187275 (Publication Type: Journal Article)

Chemical References

• DNA, Neoplasm
• Immunoglobulin Heavy Chains
• Receptors, Antigen, T-Cell, gamma-delta

Topics

• B-Lymphocytes
• Case-Control Studies
• Clone Cells (pathology)
• DNA, Neoplasm (analysis, blood, genetics)
• Gene Rearrangement
• Humans
• Immunoglobulin Heavy Chains (genetics)
• Lymphoproliferative Disorders (diagnosis, genetics, pathology)
• Plasma
• Receptors, Antigen, T-Cell, gamma-delta (genetics)
• T-Lymphocytes
• Translocation, Genetic