Abstract | OBJECTIVE:
Adiponectin is a protein abundantly secreted by the adipose tissue (AT). Plasma adiponectin levels are decreased in obese, insulin-resistant, and type 2 diabetic patients. Various multimeric complexes, i.e. high-, middle-, and low-molecular weight isoforms (HMW, MMW and LMW), are present in plasma. Here, we investigated the effect of weight reducing diet on the distribution of adiponectin isoforms in plasma and on their secretion in AT explants from obese subjects. DESIGN: A total of 20 obese subjects (age 37.8+/-7.3 years, body mass index 33.9+/-5.0 kg/m(2)) underwent eight weeks of very low-calorie diet (VLCD). A needle biopsy of subcutaneous abdominal AT and blood samples were taken before and after dietary intervention. AT explants were incubated in culture medium for 4 h. ELISA assay and western blot analyses were used to identify adiponectin complexes in culture media and in plasma. RESULTS: CONCLUSIONS:
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Authors | Zuzana Kovacova, Michaela Vitkova, Michaela Kovacikova, Eva Klimcakova, Magda Bajzova, Zuzana Hnevkovska, Lenka Rossmeislova, Vladimir Stich, Dominique Langin, Jan Polak |
Journal | European journal of endocrinology
(Eur J Endocrinol)
Vol. 160
Issue 4
Pg. 585-92
(Apr 2009)
ISSN: 1479-683X [Electronic] England |
PMID | 19155318
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adiponectin
- Culture Media
- Polymers
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Topics |
- Adiponectin
(chemistry, metabolism)
- Adipose Tissue
(metabolism)
- Adult
- Biopsy, Needle
- Blotting, Western
- Body Height
(physiology)
- Body Weight
(physiology)
- Caloric Restriction
- Culture Media
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Kinetics
- Luminescence
- Male
- Molecular Weight
- Obesity
(diet therapy, metabolism)
- Polymers
(chemistry, metabolism)
- Tissue Culture Techniques
- Waist-Hip Ratio
- Weight Loss
(physiology)
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