[Study on Porphyromonas gingivalis colonization in patients with periodontitis after periodontal initial therapy].

Abstract

OBJECTIVE:

To examine Porphyromonas gingivalis (Pg) in subgingival plaque of the patients with periodontitis and to find out the rules of Pg colonization after periodontal initial treatment.

METHODS:

A total of 1620 subgingival plaque samples were collected from 180 subjects including chronic periodontitis (CP) patients (n = 90), and aggressive periodontitis (AgP) patients (n = 90) in different periods of periodontal initial therapy-the baseline, 6 weeks, and 12 weeks after treatment. The following periodontal clinical parameters were recorded with Florida probe at sampled sites: probing depth (PD), clinical attachment loss (CAL), and bleeding on probing (BOP). Quantities of Pg were examined by AmpliFluor endpoint quantitative polymerase chain reaction.

RESULTS:

At the 6th week of periodontal initial therapy, there were 61 (22.6%) and 66 (24.4%) Pg increased sites respectively, in which no significant difference was detected (P > 0.05). At baseline of periodontal initial therapy, more severe periodontal clinical parameters of Pg increased sites were observed than those of Pg stationary sites. At the 12th week, however, there were 96 (35.6%) and 18 (6.7%) Pg increased sites respectively, significant difference detected (P < 0.05). At 6th week of periodontal initial therapy, more severe periodontal clinical parameters of Pg increased sites were observed than those of Pg stationary sites.

CONCLUSIONS:

Pg colonization in AgP and CP patients started 6 weeks after periodontal initial therapy, but the recolonization pattern was different between these two groups of patients. Severe periodontitis sites in baseline seemed to place them at risk of Pg colonization after periodontal initial therapy.

• Authors: Jing-bo Liu, Li Lin, Ya-ping Pan, Guan-hua Wang, Chen Li
• Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (Zhonghua Kou Qiang Yi Xue Za Zhi) Vol. 43 Issue 8 Pg. 478-82 (Aug 2008) ISSN: 1002-0098 [Print] China
• PMID: 19087587 (Publication Type: Comparative Study, English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Adolescent
• Adult
• Aged
• Aggressive Periodontitis (microbiology, therapy)
• Chronic Periodontitis (microbiology, therapy)
• Female
• Humans
• Male
• Middle Aged
• Polymerase Chain Reaction
• Porphyromonas gingivalis (isolation & purification)
• Young Adult