Abstract |
The whole-blood interferon-gamma enzyme-linked immunosorbent assay (QuantiFERON-TB Gold [QFT-G]; Cellestis, Carnegie, Australia) has been studied mainly for diagnosing active pulmonary tuberculosis (TB) or latent TB. We prospectively evaluated its diagnostic usefulness in patients suspected with extrapulmonary TB (EP-TB). Of the 100 adult patients with suspected EP-TB, 43 were classified as "confirmed" EP-TB and 5 as "probable" EP-TB. Of the 48 with EP-TB, 27 (56%) were diagnosed with TB lymphadenitis and 11 (17%) with skeletal TB. The overall sensitivity and specificity of the assay were 69% (95% confidence interval [CI(95)], 53-81%) and 82% (CI(95), 69-91%), respectively. Among 44 patients presented with cervical lymphadenopathy, the QFT-G assay showed 86% (CI(95), 64-97%) sensitivity and 87% (CI(95), 66-97%) specificity, whereas in 28 with skeletal involvement, the sensitivity and specificity of the assay were 45% (CI(95), 17-77%) and 81% (CI(95), 54-96%), respectively. These suboptimal diagnostic performances suggest that the QFT-G assay alone is not sufficient for the diagnosis of EP-TB.
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Authors | Kyoung-Ho Song, Jae Hyun Jeon, Wan Beom Park, Sung-Han Kim, Kyoung Un Park, Nam Joong Kim, Myoung-don Oh, Hong Bin Kim, Kang Won Choe |
Journal | Diagnostic microbiology and infectious disease
(Diagn Microbiol Infect Dis)
Vol. 63
Issue 2
Pg. 182-7
(Feb 2009)
ISSN: 1879-0070 [Electronic] United States |
PMID | 19070449
(Publication Type: Journal Article)
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Chemical References |
- Reagent Kits, Diagnostic
- Interferon-gamma
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Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Enzyme-Linked Immunosorbent Assay
(methods)
- Female
- Humans
- Interferon-gamma
(blood)
- Male
- Middle Aged
- Mycobacterium tuberculosis
(genetics, isolation & purification)
- Polymerase Chain Reaction
- Predictive Value of Tests
- Reagent Kits, Diagnostic
- Sensitivity and Specificity
- Tuberculosis
(diagnosis, immunology, microbiology)
- Tuberculosis, Lymph Node
(diagnosis, immunology, microbiology)
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