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Usefulness of the whole-blood interferon-gamma release assay for diagnosis of extrapulmonary tuberculosis.

Abstract
The whole-blood interferon-gamma enzyme-linked immunosorbent assay (QuantiFERON-TB Gold [QFT-G]; Cellestis, Carnegie, Australia) has been studied mainly for diagnosing active pulmonary tuberculosis (TB) or latent TB. We prospectively evaluated its diagnostic usefulness in patients suspected with extrapulmonary TB (EP-TB). Of the 100 adult patients with suspected EP-TB, 43 were classified as "confirmed" EP-TB and 5 as "probable" EP-TB. Of the 48 with EP-TB, 27 (56%) were diagnosed with TB lymphadenitis and 11 (17%) with skeletal TB. The overall sensitivity and specificity of the assay were 69% (95% confidence interval [CI(95)], 53-81%) and 82% (CI(95), 69-91%), respectively. Among 44 patients presented with cervical lymphadenopathy, the QFT-G assay showed 86% (CI(95), 64-97%) sensitivity and 87% (CI(95), 66-97%) specificity, whereas in 28 with skeletal involvement, the sensitivity and specificity of the assay were 45% (CI(95), 17-77%) and 81% (CI(95), 54-96%), respectively. These suboptimal diagnostic performances suggest that the QFT-G assay alone is not sufficient for the diagnosis of EP-TB.
AuthorsKyoung-Ho Song, Jae Hyun Jeon, Wan Beom Park, Sung-Han Kim, Kyoung Un Park, Nam Joong Kim, Myoung-don Oh, Hong Bin Kim, Kang Won Choe
JournalDiagnostic microbiology and infectious disease (Diagn Microbiol Infect Dis) Vol. 63 Issue 2 Pg. 182-7 (Feb 2009) ISSN: 1879-0070 [Electronic] United States
PMID19070449 (Publication Type: Journal Article)
Chemical References
  • Reagent Kits, Diagnostic
  • Interferon-gamma
Topics
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Female
  • Humans
  • Interferon-gamma (blood)
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis (genetics, isolation & purification)
  • Polymerase Chain Reaction
  • Predictive Value of Tests
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Tuberculosis (diagnosis, immunology, microbiology)
  • Tuberculosis, Lymph Node (diagnosis, immunology, microbiology)

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