Cause of hematic cysts of the orbit: increased fibrinolysis and immunohistologic expression of tissue plasminogen activator.

Abstract

PURPOSE:

Hematic cysts of the orbit are relatively uncommon. These cysts expand gradually, leading to progressive orbital symptoms. To clarify the cause of hematic cyst, especially the mechanisms leading to its gradual expansion, we investigated the immunohistologic expression of tissue-type plasminogen activator (tPA), a key enzyme of fibrinolysis, in cases of hematic cysts.

DESIGN:

Retrospective small case series.

PARTICIPANTS:

Three patients with hematic cyst of the orbit were studied.

METHODS:

Three surgically removed hematic cysts were studied from a histologic perspective, including immunohistochemistry for tPA. The cyst content was also analyzed chemically.

MAIN OUTCOME MEASURES:

The pathologic features of hematic cyst of the orbit, expression of tPA in the cyst wall, and coagulation-fibrinolytic factors in the content of the cyst.

RESULTS:

The cyst wall was composed of dense collagen fibers lacking an epithelial lining and contained many fine capillary- or venule-like vessels. Hemosiderin-laden macrophages were observed among the collagen fibers. Strong immunoreactivity for tPA was revealed in the endothelial cells of the vessels in the cyst wall. Chemical analysis of the cyst content revealed a marked delay in the activated partial thromboplastin time and prothrombin time, a low fibrinogen concentration, and high concentrations of the D-dimer and tPA-plasminogen activator inhibitor-1 complex.

CONCLUSIONS:

Our findings indicate that blood coagulation and hemostasis occur first and that fibrinolysis occurs second in hematic cysts. Gradual expansion of the cyst may be due to hyperfibrinolysis resulting from oversecretion of tPA from the endothelial cells in the cyst wall, thus impairing normal hemostasis. Hemorrhage may then recur, resulting in enlargement of the hematic cyst. These mechanisms are similar to those occurring in chronic subdural hematomas.

FINANCIAL DISCLOSURE(S):

The author(s) have no proprietary or commercial interest in any materials discussed in this article.

• Authors: Koichi Yoshikawa, Hirosuke Fujisawa, Koji Kajiwara, Masami Fujii, Shoichi Kato, Tatsuo Akimura, Sadahiro Nomura, Toshikazu Gondo, Michiyasu Suzuki
• Journal: Ophthalmology (Ophthalmology) Vol. 116 Issue 1 Pg. 130-4 (Jan 2009) ISSN: 1549-4713 [Electronic] United States
• PMID: 19019445 (Publication Type: Case Reports, Journal Article)

Chemical References

• Fibrinolytic Agents
• Plasminogen Activator Inhibitor 1
• SERPINE1 protein, human
• Tissue Plasminogen Activator

Topics

• Aged
• Cysts (diagnosis, etiology, metabolism)
• Endothelium, Vascular (metabolism)
• Female
• Fibrinolysis
• Fibrinolytic Agents (metabolism)
• Hematoma (diagnosis, etiology, metabolism)
• Humans
• Immunoenzyme Techniques
• Magnetic Resonance Imaging
• Male
• Middle Aged
• Orbital Diseases (diagnosis, etiology, metabolism)
• Partial Thromboplastin Time
• Plasminogen Activator Inhibitor 1 (analysis)
• Prothrombin Time
• Retrospective Studies
• Tissue Plasminogen Activator (metabolism)
• Tomography, X-Ray Computed