Quantitation of steroid hormones in thin fresh frozen tissue sections.

Abstract

As analytical technologies in proteomics and metabolomics continue to mature, there is an increasing need to apply these to clinically relevant biologic samples. In this study, a liquid chromatography-tandem mass spectrometry method that utilizes selected reaction monitoring was used to measure the absolute quantity of estrogens and estrogen metabolites and testosterone in 8-microm tissue sections obtained from a fresh frozen lymph node tumor infiltrated by metastatic breast carcinoma. Total (conjugated plus unconjugated) and unconjugated levels of these steroid hormones were measured using two cohorts, each containing five adjacent serial sections cut from this tumor. The results were highly reproducible across replicate samples, showing that typical histological tissue sections represent an important sample type for the measurement of these specific metabolites.

• Authors: Josip Blonder, Donald J Johann, Timothy D Veenstra, Zhen Xiao, Michael R Emmert-Buck, Regina G Ziegler, Jaime Rodriguez-Canales, Jeffrey A Hanson, Xia Xu
• Journal: Analytical chemistry (Anal Chem) Vol. 80 Issue 22 Pg. 8845-52 (Nov 15 2008) ISSN: 1520-6882 [Electronic] United States
• PMID: 18937426 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural)

Chemical References

• Estrogens
• Hormones
• Steroids
• Testosterone

Topics

• Cell Line, Tumor
• Chromatography, Liquid
• Estrogens (analysis)
• Frozen Sections
• Hormones (analysis)
• Humans
• Steroids (analysis)
• Tandem Mass Spectrometry
• Testosterone (analysis)