Adeno-associated virus type 2 vectors: transduction and long-term expression in cerebellar Purkinje cells in vivo is mediated by the fibroblast growth factor receptor 1 : bFGFR-1 mediates AAV2 transduction of Purkinje cells.

Abstract

The receptor for adeno-associated virus serotype 2 (AAV2) in Purkinje cells has not been identified, but based on work carried out in non-neuronal cell lines, heparan sulfate proteoglycan is thought to act as a primary receptor, with basic fibroblast growth factor receptor-1 being reported as a co-receptor. In this study, using antibody interference and protein competition strategies, we show specific reduction in Purkinje cell transduction by AAV2 vector in the presence of these inhibitors. We also demonstrate AAV2-mediated transgene expression in Purkinje cells in vivo that extends out to one year post-injection. These results provide new information on fibroblast growth factor receptor-1 involvement in AAV2-mediated transduction of Purkinje cells and have important implications for AAV-mediated treatment of various cerebellar disorders.

• Authors: Lalitha R Belur, William F Kaemmerer, R Scott McIvor, Walter C Low
• Journal: Archives of virology (Arch Virol) Vol. 153 Issue 11 Pg. 2107-10 ( 2008) ISSN: 1432-8798 [Electronic] Austria
• PMID: 18937030 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Receptor, Fibroblast Growth Factor, Type 1

Topics

• Animals
• Brain (metabolism, virology)
• Dependovirus (genetics, metabolism)
• Female
• Gene Expression
• Gene Transfer Techniques
• Genetic Vectors (genetics)
• Mice
• Purkinje Cells (metabolism, virology)
• Receptor, Fibroblast Growth Factor, Type 1 (genetics, metabolism)
• Transduction, Genetic