Effect of sodium tanshinone II A sulfonate on cardiac myocyte hypertrophy and its underlying mechanism.

Abstract

OBJECTIVE:

To investigate the effects of sodium tanshinone II A sulfonate (STS) on the hypertrophy induced by angiotensin II (Ang II) in primary cultured neonatal rat cardiac myocytes.

METHODS:

The effect of STS on cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-3,5-phenytetrazoliumromide (MTT) assay. As indexes for cardiocyte hypertrophy, cell size was determined by phase contrast microscopy and protein synthesis rate was measured by 3H-leucine incorporation. The proto-oncogene c-fos mRNA expression of cardiocytes was assessed using reverse transcription polymerase chain reaction (RT-PCR).

RESULTS:

STS could inhibit cardiocyte hypertrophy, increase the protein synthesis rate and enhance proto-oncogene c-fos mRNA expression in cardiocytes induced by Ang II (P<0.01), with an effect similar to that of Valsartan, the Ang II receptor antagonist.

CONCLUSION:

STS can prevent the hypertrophy of cardiac myocytes induced by Ang II, which may be related to its inhibition of the expression of proto-oncogene c-fos mRNA.

• Authors: Jun Feng, Zhi Zheng
• Journal: Chinese journal of integrative medicine (Chin J Integr Med) Vol. 14 Issue 3 Pg. 197-201 (Sep 2008) ISSN: 1672-0415 [Print] China
• PMID: 18853116 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phenanthrenes
• Proto-Oncogene Proteins c-fos
• RNA, Messenger
• Tritium
• Angiotensin II
• tanshinone II A sodium sulfonate
• Leucine

Topics

• Angiotensin II (pharmacology)
• Animals
• Animals, Newborn
• Cell Survival (drug effects)
• Gene Expression Regulation (drug effects)
• Hypertrophy
• Leucine (metabolism)
• Myocytes, Cardiac (drug effects, pathology)
• Phenanthrenes (pharmacology)
• Proto-Oncogene Proteins c-fos (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)
• Rats
• Rats, Wistar
• Time Factors
• Tritium