Effect of MMP-1 promoter polymorphisms on GCF MMP-1 levels and outcome of periodontal therapy in patients with severe chronic periodontitis.

Abstract

AIMS:

The aims of this study were to investigate (1) the matrix metalloproteinase-1 (MMP-1) promoter polymorphisms in severe chronic periodontitis (CP), (2) the relationship of periodontal therapy outcome with these genotypes, and (3) the gingival crevicular fluid (GCF) MMP-1 levels-MMP-1 genotype correlation.

MATERIAL AND METHODS:

Genomic DNA was obtained from the peripheral blood of 102 patients with severe CP and 98 periodontally healthy subjects. MMP-1 -519A/G and -1607 1G/2G polymorphisms were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Fifty-eight CP patients received non-surgical periodontal therapy and were followed for 6 months. Clinical periodontal parameters and GCF samples were collected at baseline and at 6 months. GCF MMP-1 levels were analysed by enzyme-linked immunosorbent assay (ELISA).

RESULTS:

The distribution of MMP-1 genotypes did not significantly differ between the study groups. On the other hand, the -1607 2G allele frequency of severe CP patients was higher than that of healthy subjects. MMP-1 -519G allele carriers had higher GCF MMP-1 levels and percentage of sites with 4-6 mm clinical attachment level (CAL) compared with AA genotypes after non-surgical periodontal therapy (p<0.05).

CONCLUSIONS:

These data suggest that the -1607 2G polymorphic allele of the MMP-1 gene could be associated with susceptibility to severe CP in the Turkish population. It seems that -519AG and GG genotypes could play a role in the outcome of periodontal therapy.

• Authors: Demet Pirhan, Gül Atilla, Gülnur Emingil, Timo Sorsa, Taina Tervahartiala, Afig Berdeli
• Journal: Journal of clinical periodontology (J Clin Periodontol) Vol. 35 Issue 10 Pg. 862-70 (Oct 2008) ISSN: 1600-051X [Electronic] United States
• PMID: 18710412 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Matrix Metalloproteinase 1

Topics

• Adult
• Aged
• Alleles
• Case-Control Studies
• Chi-Square Distribution
• Chronic Periodontitis (enzymology, genetics, therapy)
• Dental Scaling
• Female
• Gene Frequency
• Gingival Crevicular Fluid (enzymology)
• Humans
• Male
• Matrix Metalloproteinase 1 (genetics, metabolism)
• Middle Aged
• Periodontal Index
• Polymorphism, Genetic
• Promoter Regions, Genetic (genetics)
• Reference Values
• Severity of Illness Index
• Treatment Outcome