Genetic variants in protein kinase C zeta gene and type 2 diabetes risk: a case-control study of a Chinese Han population.

Abstract

BACKGROUND:

Previous investigations of the protein kinase C zeta (PRKCZ) gene, a susceptibility factor for type 2 diabetes mellitus (T2DM), have focussed on its single nucleotide polymorphisms (SNPs). In a departure from these earlier studies, we tested six tagging markers of PRKCZ based on the Hapmap database to evaluate their association with T2DM.

METHODS:

PCR-RFLP was performed to test the genotypes in type 2 diabetes patients (n = 343) and normal controls (n = 429). Insulin-related indexes were calculated from levels of fasting plasma insulin (FPI) and fasting plasma glucose (FPG).

RESULTS:

The variant genotypes, rs3753242TT, rs2503706CT, rs2503706CT/ TT and rs262654TT were associated with T2DM. And the significance remained for rs3753242 TT (p < 0.001) after the false discovery rate (FDR) correction. There was a doubling in the prevalence of the TTGCCC haplotype in the case subjects (14.2%) compared with the control subjects (6.9%) [OR (95% CI) = 2.19(1.49-3.22)]. In the control group, none of the haplotypes studied was associated with insulin-related indexes.

CONCLUSIONS:

The PRKCZ gene variants associated with the development of T2DM in this study must be investigated in a larger population to reveal any potential effects on metabolism.

• Authors: Lei Qin, Ling Zhou, Xueji Wu, Jinluo Cheng, Jingsong Wang, Yunfeng Du, Jihong Hu, Moyu Shen, Jinyi Zhou
• Journal: Diabetes/metabolism research and reviews (Diabetes Metab Res Rev) Vol. 24 Issue 6 Pg. 480-5 (Sep 2008) ISSN: 1520-7552 [Print] England
• PMID: 18615853 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• protein kinase C zeta
• Protein Kinase C

Topics

• Asian People (genetics)
• Case-Control Studies
• China
• DNA Primers
• Diabetes Mellitus, Type 2 (enzymology, epidemiology, genetics, metabolism)
• Genetic Predisposition to Disease
• Genetic Variation
• Genotype
• Humans
• Interviews as Topic
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Polymorphism, Single Nucleotide
• Protein Kinase C (genetics)
• Risk Factors
• Surveys and Questionnaires