Combined stimulation of nasal polyp fibroblasts with poly IC, interleukin 4, and tumor necrosis factor alpha potently induces production of thymus- and activation-regulated chemokine.

Abstract

OBJECTIVE:

To examine the effects of cytokines and poly IC on the expression of thymus- and activation-regulated chemokine (TARC), a potent chemoattractant for helper T-cell type 2 (T(H)2) cells, in nasal polyp fibroblasts.

DESIGN:

Quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis.

SETTING:

Academic research.

PARTICIPANTS:

Primary fibroblast lines were established from human nasal polyp biopsy tissue specimens (n = 5) removed at polypectomy.

MAIN OUTCOME MEASURES:

The expression of TARC messenger RNA (mRNA) was evaluated by real-time RT-PCR. The amount of TARC in the supernatants was measured by enzyme-linked immunosorbent assay.

RESULTS:

Combined stimulation with interleukin 4 (IL-4) and tumor necrosis factor alpha (TNF-alpha) or with poly IC and IL-4 induced TARC production. Combined exposure of cells to poly IC, IL-4, and TNF-alpha resulted in substantial amounts of TARC release into the culture medium. Quantitative RT-PCR analysis revealed that simultaneous stimulation with those 3 compounds induced a tremendous increase in the amount of TARC mRNA in the nasal polyp fibroblasts.

CONCLUSION:

Nasal polyp fibroblasts contribute to T(H)2 cell infiltration and RNA virus-induced exacerbation of T(H)2-type airway inflammatory conditions such as allergic chronic sinusitis.

• Authors: Manabu Nonaka, Nozomu Ogihara, Akira Fukumoto, Atsuko Sakanushi, Ruby Pawankar, Toshiaki Yagi
• Journal: Archives of otolaryngology--head & neck surgery (Arch Otolaryngol Head Neck Surg) Vol. 134 Issue 6 Pg. 630-5 (Jun 2008) ISSN: 1538-361X [Electronic] United States
• PMID: 18559731 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• CCL17 protein, human
• Chemokine CCL17
• Tumor Necrosis Factor-alpha
• Interleukin-4
• Poly I-C

Topics

• Adjuvants, Immunologic (pharmacology)
• Cells, Cultured
• Chemokine CCL17 (biosynthesis)
• Fibroblasts (drug effects, immunology)
• Humans
• Interleukin-4 (pharmacology)
• Nasal Polyps (immunology)
• Poly I-C (pharmacology)
• Tumor Necrosis Factor-alpha (pharmacology)