Abstract | OBJECTIVES: DESIGN AND METHODS: Levels and activities of plasma MMP-2 and MMP-9 were investigated by enzyme-linked immunosorbent assay and zymography, respectively. RESULTS: MMP-2 levels in control subjects, RA and SLE patients were 146.1+/-34.2, 194.0+/-24.2 and 208.9+/-75.9 ng/mL respectively, and for MMP-9 were 51.4+/-57.1, 567.7+/-313.1 and 208.7+/-105.5 ng/mL respectively. Both MMP-2 and MMP-9 levels and activities from all patients were significantly higher than that from control subjects. CONCLUSIONS: MMP-2 levels in both patients groups were approximately 1.3-1.4 folds higher than that in control subjects, notably, MMP-9 levels were 11- and 4-folds significantly higher, respectively, in RA and SLE patients. The results which MMP-2 and MMP-9 levels and activities are significantly elevated support the involvement of MMPs proteins in these autoimmune disorders.
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Authors | Yih-Hsin Chang, I-Ling Lin, Gregory J Tsay, Shun-Chun Yang, Tzi-Peng Yang, Kuo-Ting Ho, Tsai-Ching Hsu, Ming-Yuh Shiau |
Journal | Clinical biochemistry
(Clin Biochem)
Vol. 41
Issue 12
Pg. 955-9
(Aug 2008)
ISSN: 1873-2933 [Electronic] United States |
PMID | 18471998
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MMP2 protein, human
- Matrix Metalloproteinase 2
- Matrix Metalloproteinase 9
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Topics |
- Adolescent
- Adult
- Arthritis, Rheumatoid
(enzymology)
- Electrophoresis, Polyacrylamide Gel
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Lupus Erythematosus, Systemic
(enzymology)
- Male
- Matrix Metalloproteinase 2
(blood)
- Matrix Metalloproteinase 9
(blood)
- Middle Aged
- Taiwan
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