Utilization of fluorescence tracer in hyperinsulinemic-euglycemic clamp test in mice.

Abstract

The hyperinsulinemic-euglycemic clamp test is considered to be a gold standard for the evaluation of insulin sensitivity. Here, a new version of the clamp test that used the fluorescence tracer 2-NBDG was tested. C57BL/6J mice were induced insulin resistant (IR) with a high-calorie diet. Rosiglitazone was administrated to IR mice and diabetic db/db mice. Insulin resistance was estimated with the oral glucose tolerance test (OGTT), the insulin tolerance test (ITT), the serum insulin level and the homeostasis model assessment of insulin resistance (HOMA-IR), and then confirmed by the hyperinsulinemic-euglycemic clamp test with 2-NBDG. The 2-NBDG content was measured by the fluorescence intensity. The characteristics of insulin resistance were shown remarkably with the increased values of serum insulin and HOMA-IR in IR mice, and with the results from OGTT and ITT in both IR and db/db mice. In the hyperinsulinemic-euglycemic clamp test, the glucose infusion rate and amount of 2-NBDG taken up into the liver, adipose, and skeletal muscle were decreased significantly in IR mice and db/db mice, respectively. The clearing rates of 2-NBDG from the circulation were much slower in both mouse models. All markers were reversed significantly by rosiglitazone treatment. The results indicate that with the fluorescence tracer 2-NBDG, the hyperinsulinemic-euglycemic clamp test can be used to estimate insulin sensitivity in vivo.

• Authors: Fei Ye, Rongya Tao, Weina Cong, Jinying Tian, Qian Liu
• Journal: Journal of biochemical and biophysical methods (J Biochem Biophys Methods) Vol. 70 Issue 6 Pg. 978-84 (Apr 24 2008) ISSN: 0165-022X [Print] Netherlands
• PMID: 18355922 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Glucose
• Fluorescent Dyes
• Deoxyglucose
• 4-Chloro-7-nitrobenzofurazan
• 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose

Topics

• 4-Chloro-7-nitrobenzofurazan (analogs & derivatives, analysis)
• Animal Feed
• Animals
• Blood Glucose (metabolism)
• Deoxyglucose (analogs & derivatives, analysis)
• Female
• Fluorescent Dyes (analysis)
• Glucose Clamp Technique (methods)
• Hyperinsulinism (blood)
• Insulin Resistance
• Male
• Mice
• Mice, Inbred C57BL
• Organ Specificity