Expression of pancreatitis associated proteins in urothelium and urinary afferent neurons following cyclophosphamide induced cystitis.

Abstract

PURPOSE:

We examined the expression profile of the members of the pancreatitis associated proteins/regenerating gene family in the bladder and in the primary afferent neurons of dorsal root ganglia using an animal model of cystitis.

MATERIALS AND METHODS:

We examined the expression of pancreatitis-associated protein-I and pancreatitis-associated protein-III in the bladder and the dorsal root ganglia of female rats 4 hours, 48 hours or 10 days after cyclophosphamide (Sigma) injection using immunohistochemistry and reverse transcriptase-polymerase chain reaction.

RESULTS:

No pancreatitis-associated protein-III immunoreactivity was identified in control bladders but prominent expression was observed in the urothelium of animals with chronic cystitis. Cells expressing pancreatitis-associated protein-I were seen in the dorsal root ganglia but not in the bladder. In normal dorsal root ganglia pancreatitis-associated protein-I was expressed in a minor population of small diameter neurons (2.4%) that were also positive for isolectin-B4. However, by 10 days following the onset of cystitis the number of pancreatitis-associated protein-I positive neurons was increased (7.6%) and pancreatitis-associated protein-I immunoreactivity was further observed in a slightly larger group of neurons and tyrosine kinase A positive small neurons.

CONCLUSIONS:

The current results suggest that pancreatitis-associated protein-III is associated with bladder inflammation and they implicate pancreatitis-associated protein-I in the abnormal sensation in cystitis.

• Authors: Yuki Takahara, Akinobu Suzuki, Mitsuyo Maeda, Hidenori Kawashima, Tatsuya Nakatani, Hiroshi Kiyama
• Journal: The Journal of urology (J Urol) Vol. 179 Issue 4 Pg. 1603-9 (Apr 2008) ISSN: 1527-3792 [Electronic] United States
• PMID: 18295254 (Publication Type: Journal Article)

Chemical References

• Antigens, Neoplasm
• Biomarkers, Tumor
• Lectins, C-Type
• Noxae
• Pancreatitis-Associated Proteins
• REG3A protein, human
• Reg3b protein, rat
• Reg3g protein, rat
• Cyclophosphamide

Topics

• Animals
• Antigens, Neoplasm (genetics)
• Biomarkers, Tumor (genetics)
• Cyclophosphamide (adverse effects)
• Cystitis (chemically induced, genetics, physiopathology)
• Disease Models, Animal
• Female
• Ganglia, Spinal (metabolism)
• Gene Expression Profiling
• Lectins, C-Type (genetics)
• Neurons, Afferent (metabolism)
• Noxae (adverse effects)
• Pancreatitis-Associated Proteins
• Rats
• Rats, Sprague-Dawley
• Urothelium (metabolism)