Reproducibility of serum leptin, insulin-like growth factor-I, and insulin-like growth factor-binding protein-3 measurements.

Abstract

BACKGROUND:

Many epidemiologic studies examine the effects of single measurements of hormones, including those related to obesity such as insulin-like growth factors (IGFs) or leptin, on disease associations; however, few studies have determined whether these single values accurately reflect measurements over time.

METHODS:

We examined the reproducibility of hormones associated with obesity, specifically leptin, IGF-I, and IGF-binding protein-3 (IGFBP-3). Two fasting blood samples, approximately four months apart, were collected from 38 participants from the Seattle metropolitan area; leptin, IGF-I, and IGFBP-3 concentrations were measured in previously unthawed serum samples. Unadjusted and adjusted intraclass correlation coefficients (ICC) were calculated to assess reproducibility.

RESULTS:

Adjusting for age and sex, the ICCs for leptin, IGF-I, and IGFBP-3 were 0.73, 0.83 and 0.60, respectively. Weighted kappas yielded similar results.

CONCLUSION:

These data suggest that for leptin, IGF-I, and IGFBP-3, a single fasting serum measurement can fairly reliably reflect the hormonal milieu over periods of months.

• Authors: Victoria M Chia, Polly A Newcomb, Emily White, Yingye Zheng, John D Potter, Johanna W Lampe
• Journal: Hormone research (Horm Res) Vol. 69 Issue 5 Pg. 295-300 ( 2008) ISSN: 1423-0046 [Electronic] Switzerland
• PMID: 18259109 (Publication Type: Evaluation Study, Journal Article, Research Support, N.I.H., Extramural)
• Copyright: (c) 2008 S. Karger AG, Basel

Chemical References

• Insulin-Like Growth Factor Binding Protein 3
• Leptin
• Insulin-Like Growth Factor I

Topics

• Adult
• Body Weight
• Fasting (blood)
• Female
• Follow-Up Studies
• Humans
• Insulin-Like Growth Factor Binding Protein 3 (analysis, blood)
• Insulin-Like Growth Factor I (analysis)
• Leptin (analysis, blood)
• Male
• Randomized Controlled Trials as Topic
• Reproducibility of Results
• Research Design