The antibody preparation and expression of human Pescadillo.

Abstract

To explore the biological roles of human Pescadillo and investigate its potential effect on tumorigenesis, the cDNA of Pescadillo was fused with that of GST. After purification and elution, the purified GST-Pescadillo fusion protein was obtained, and the antibody against the fusion protein was generated. Endogenous Pescadillo protein was observed to be remarkably induced by estrogen. It was mainly distributed in the tissues such as breast, ovary and intestine, all of which contain proliferating cells, and was also detected in many cell lines of human cancer: renal carcinoma, hepatoma, ovarian cancer, colon carcinoma, and breast cancer. The expression level of Pescadillo was increased significantly in breast cancer tissues compared with their paired margin tissues. Taken together, these data suggest that Pescadillo may play important roles in the initiation and development of cancer and may be a potential target in cancer diagnosis and therapy.

• Authors: Hao Zhang, JiePing Li, XiaoHui Wang, Yan Sun, Bin Yuan, ZhiHong Yang, YanChao Jiang, Min Zeng, LiHua Ding, Kang Ning, JianHua Zhu, JieZhi Li, CuiFen Huang, AiJun Liu, QiNong Ye
• Journal: Science in China. Series C, Life sciences (Sci China C Life Sci) Vol. 50 Issue 3 Pg. 298-304 (Jun 2007) ISSN: 1006-9305 [Print] China
• PMID: 17609885 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• PES1 protein, human
• Proteins
• RNA-Binding Proteins
• Recombinant Fusion Proteins

Topics

• Animals
• Antibodies
• Antibody Specificity
• Breast
• Breast Neoplasms (genetics)
• COS Cells
• Cell Line, Tumor
• Chlorocebus aethiops
• Female
• Humans
• Mice
• Mice, Inbred BALB C
• Proteins (genetics, immunology, metabolism)
• RNA-Binding Proteins
• Recombinant Fusion Proteins (immunology, metabolism)