Abstract | BACKGROUND: METHODS: BALB/c mice were repeatedly exposed to hazelnut protein via the transdermal route and systemic allergic and anaphylactic responses were studied. RESULTS: Transdermal exposure to hazelnut protein elicited robust systemic IgE response in a dose-dependent manner with immunological memory. Oral challenge of transdermally sensitized mice with hazelnut protein resulted in immediate (30 min after the challenge) clinical signs of systemic anaphylaxis as measured by significant clinical scores and drop in rectal temperature. Clinical hypersensitivity reaction was associated with severe pathological changes in the small intestine. Hazelnut-allergic but not control mice exhibited in vivo activation of GATA-3 and hazelnut-driven recall IL-4, IL-5 and IL-13 response by splenocytes, thus elucidating the underlying mechanism of hazelnut allergy development in this model. CONCLUSIONS:
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Authors | Neil Patrick Birmingham, Sitaram Parvataneni, Hanem M Ahmed Hassan, Jack Harkema, Sridhar Samineni, Lalitha Navuluri, Caleb James Kelly, Venu Gangur |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 144
Issue 3
Pg. 203-10
( 2007)
ISSN: 1423-0097 [Electronic] Switzerland |
PMID | 17570928
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | 2007 S. Karger AG, Basel |
Chemical References |
- Adjuvants, Immunologic
- Immunoglobulin E
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Topics |
- Adjuvants, Immunologic
- Anaphylaxis
(immunology)
- Animals
- Cells, Cultured
- Corylus
(immunology)
- Disease Models, Animal
- Female
- Food Hypersensitivity
(immunology, pathology)
- Immunoglobulin E
(biosynthesis)
- Immunologic Memory
- Mice
- Mice, Inbred BALB C
- Seeds
(immunology)
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