Identification of nuclear factor-kappaB responsive element within the neuronal nitric oxide synthase exon 1f-specific promoter.
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| Abstract |
Transcriptional regulation of the neuronal nitric oxide synthase gene (nNOS) is particularly complex as 12 distinct transcripts derived from different first exons are expressed in a tissue- and cell-specific manner. The exon 1f mRNA is relatively highly expressed in nervous system and relies upon exon 1f-specific promoter activity. Using conventional and real-time reverse transcription-polymerase chain reaction, we found exon 1f mRNA was the major transcript of the nNOS gene in human neuroblastoma SK-N-SH cells. We analyzed a 1090 bp fragment of 1f promoter by TRANSFAC-TESS and Match softwares and luciferase assay, and found an important positive transcriptional regulation region that contained a putative nuclear factor (NF)-kappaB binding site. Subsequently, using electrophoresis mobility shift and chromatin immunoprecipitation assays, we identified this site to be the NF-kappaB responsive element, a crucial positive regulator in the activation of the nNOS 1f promoter. Taken together, our study identified an NF-kappaB responsive element within nNOS 1f promoter and showed that it plays an important role in the transactivation of nNOS 1f mRNA, the major transcript of nNOS in SK-N-SH cells.
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| Authors | Yinghui Li, Guangyu Li, Chunyi Li, Yanyan Zhao |
| Journal | Acta biochimica et biophysica Sinica (Acta Biochim Biophys Sin (Shanghai)) Vol. 39 Issue 4 Pg. 247-54 (Apr 2007) ISSN: 1672-9145 [Print] China |
| PMID | 17417679 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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