[The determination of amylo-1,6-glucosidase in biopsy specimens from human chorion].

Abstract

This method has been developed for the prenatal diagnosis of a grave hereditary disease, a deficiency of amylo-1,6-glucosidase (A-1,6-G) activity. The methods suggested earlier did not rule out the effects of other glucosidases, that could result in false-positive data if the fetus was involved. The new method for measuring A-1,6-G activity includes sedimentation of acid alpha-glucosidase with rabbit blood antiserum to human placental acid alpha-glucosidase. A-1,6-G activity is estimated from the difference in glucose accrement with limit dextrin and glycogen used as substrates. A-1,6-G activities were measured in 15 chorion samples obtained in the first trimester of pregnancy by the developed method, and normal values of this parameter established, making up 39.77 +/- 8.1 nmol/h per g of protein. The method may be useful in the prenatal diagnosis of type III glycogenosis.

• Authors: E V Kazlas, I V Chibisov
• Journal: Laboratornoe delo (Lab Delo) Issue 5 Pg. 32-4 ( 1991) ISSN: 0023-6748 [Print] Russia (Federation)
• Vernacular Title: Opredelenie amilo-1,6-gliukozidazy v bioptate khoriona cheloveka.
• PMID: 1715938 (Publication Type: English Abstract, Journal Article)

Chemical References

• Glucan 1,4-alpha-Glucosidase

Topics

• Biopsy
• Chorion (enzymology, pathology)
• Female
• Glucan 1,4-alpha-Glucosidase (analysis)
• Glycogen Storage Disease Type III (diagnosis)
• Humans
• Pregnancy
• Prenatal Diagnosis