No interplay between the pathways mediating coagulation and inflammation in tissue factor-induced disseminated intravascular coagulation in rats.

Abstract

OBJECTIVE:

Previous reports have suggested an interplay between the pathways mediating coagulation and inflammation in endotoxemia and sepsis. The present study was designed to examine whether cross-signaling between the pathways mediating coagulation and inflammation occurs, as suggested by the pattern of cytokine production observed following tissue-factor (TF)-induced disseminated intravascular coagulation (DIC).

DESIGN:

Prospective, comparative, experimental study.

SETTING:

Laboratory at a university hospital.

SUBJECTS:

Male Wistar rats, aged 6-7 wks, and weighing 160-170 g.

INTERVENTIONS:

Male Wistar rats were administered TF (3.75 units/kg every 4 hrs), TF, and tranexamic acid (TA; 50 mg/kg every 4.5 hrs) or lipopolysaccharide (30 mg/kg every 4 hrs) via the tail vein, and blood was sampled at 0, 4, 8 and 12 hrs.

MEASUREMENTS AND MAIN RESULTS:

Subsequent alterations in thrombin-antithrombin complex and fibrinogen levels, as well as platelet counts, indicated that the severity of both types of experimental DIC (TF-induced and lipopolysaccharide-induced) was similar with respect to hemostatic activation and development of consumption coagulopathy. In lipopolysaccharide-induced DIC, a sharp increase in plasma tumor necrosis factor levels was observed at 4 hrs, after which a sharp decline was noted. Plasma levels of interleukin-6 were markedly increased at 4 hrs, after which a sustained elevation was observed for the duration of the experimental period (tumor necrosis factor, 1270 +/- 280, 180 +/- 40, and 120 +/- 30 pg/mL at 4, 8 and 12 hrs, respectively; interleukin-6, 5810 +/- 1320, 4850 +/- 730, and 5230 +/- 1280 pg/mL at 4, 8 and 12 hrs, respectively). On the other hand, tumor necrosis factor and interleukin-6 were not detected following TF-induced DIC (0 +/- 0 at 4, 8, and 12 hrs for both tumor necrosis factor and interleukin-6). In the TF+TA group, significant increases in tumor necrosis factor and interleukin-6 were observed, compared with the TF group.

CONCLUSIONS:

There is no overt interplay between the pathways mediating coagulation and inflammation in TF-induced DIC, as observed in lipopolysaccharide-induced DIC.

• Authors: Yasuo Ontachi, Hidesaku Asakura, Yoko Takahashi, Tomoe Hayashi, Masahisa Arahata, Yasuko Kadohira, Mio Maekawa, Mika Omote, Tomotaka Yoshida, Masahide Yamazaki, Eriko Morishita, Ken-ichi Miyamoto, Shinji Nakao
• Journal: Critical care medicine (Crit Care Med) Vol. 34 Issue 10 Pg. 2646-50 (Oct 2006) ISSN: 0090-3493 [Print] United States
• PMID: 16932226 (Publication Type: Comparative Study, Evaluation Study, Journal Article)

Chemical References

• Antifibrinolytic Agents
• Cytokines
• Hemostatics
• Interleukin-6
• Lipopolysaccharides
• Tumor Necrosis Factor-alpha
• Interleukin-10
• Tranexamic Acid
• Thromboplastin

Topics

• Animals
• Antifibrinolytic Agents (pharmacology)
• Cytokines (drug effects, metabolism)
• Disseminated Intravascular Coagulation (chemically induced, physiopathology)
• Hemostatics (pharmacology)
• Interleukin-10 (metabolism)
• Interleukin-6 (metabolism)
• Lipopolysaccharides (pharmacology)
• Male
• Prospective Studies
• Rats
• Rats, Wistar
• Receptor Cross-Talk (drug effects)
• Signal Transduction (drug effects)
• Thromboplastin (pharmacology)
• Tranexamic Acid (pharmacology)
• Tumor Necrosis Factor-alpha (metabolism)