Rapid, sensitive, and specific lateral-flow immunochromatographic device to measure anti-anthrax protective antigen immunoglobulin g in serum and whole blood.

Abstract

Evidence from animals suggests that anti-anthrax protective antigen (PA) immunoglobulin G (IgG) from vaccination with anthrax vaccine adsorbed (AVA) is protective against Bacillus anthracis infection. Measurement of anti-PA IgG in human sera can be performed using either enzyme-linked immunosorbent assay or fluorescent covalent microsphere immunoassay (ELISA) (R. E. Biagini, D. L. Sammons, J. P. Smith, B. A. MacKenzie, C. A. Striley, V. Semenova, E. Steward-Clark, K. Stamey, A. E. Freeman, C. P. Quinn, and J. E. Snawder, Clin. Diagn. Lab. Immunol. 11:50-55, 2004). Both these methods are laboratory based. We describe the development of a rapid lateral-flow immunochromatographic assay (LFIA) test kit for the measurement of anti-PA IgG in serum or whole-blood samples (30-microl samples) using colloidal gold nanoparticles as the detection reagent and an internal control. Using sera from 19 anthrax AVA vaccinees (anti-PA IgG range, 2.4 to 340 microg/ml) and 10 controls and PA-supplemented whole-blood samples, we demonstrated that the LFIA had a sensitivity of approximately 3 microg/ml anti-PA IgG in serum and approximately 14 microg/ml anti-PA IgG in whole blood. Preabsorption of sera with PA yielded negative anti-PA LFIAs. The diagnostic sensitivity and specificity of the assay were 100% using ELISA-measured anti-PA IgG as the standard. This kit has utility in determining anti-PA antibody reactivity in the sera of individuals vaccinated with AVA or individuals with clinical anthrax.

• Authors: Raymond E Biagini, Deborah L Sammons, Jerome P Smith, Barbara A MacKenzie, Cynthia A F Striley, John E Snawder, Shirley A Robertson, Conrad P Quinn
• Journal: Clinical and vaccine immunology : CVI (Clin Vaccine Immunol) Vol. 13 Issue 5 Pg. 541-6 (May 2006) ISSN: 1556-6811 [Print] United States
• PMID: 16682473 (Publication Type: Evaluation Study, Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Bacterial Toxins
• Colloids
• Immunoglobulin G
• anthrax toxin
• Gold

Topics

• Anthrax (blood, diagnosis, immunology)
• Antibodies, Bacterial (blood)
• Antigens, Bacterial (immunology)
• Bacillus anthracis (immunology)
• Bacterial Toxins (immunology)
• Chromatography (instrumentation, methods)
• Colloids (chemistry)
• Gold (chemistry)
• Humans
• Immunoassay (instrumentation, methods)
• Immunoglobulin G (blood)
• Nanostructures (chemistry)
• Sensitivity and Specificity
• Time Factors