Polyclonal antibody to
S-100 protein has been routinely applied for initial screening of various types of
tumors, including, melanocytic
tumors and neurogenic
tumors.
S-100 protein has been shown to have a broad distribution in human tissues, including renal tubules. The potential utility of
S-100 protein in renal cell
neoplasms has not been extensively investigated. Using an EnVision-
Horseradish Peroxidase (HRP; Dako, Carpinteria, Calif) kit, we evaluated the diagnostic value of
S-100 protein on tissue microarray sections from 175 cases of renal
epithelial neoplasm (145 primary
renal neoplasms and 30 metastatic
renal cell carcinomas) and 24 non-neoplastic renal tissues. Immunohistochemical stains for pancytokeratin, HMB-45, and Mart-1 were also performed. Western blot using the same antibody (anti-S-100 protein) was performed on 10 cases of renal cell
neoplasm. The results demonstrated that nuclear and cytoplasmic staining pattern for
S-100 protein was observed in 56 (69%) of 81 conventional (clear cell)
renal cell carcinomas (RCCs), 10 (30%) of 33 papillary RCCs, 1 (6%) of 16 ChRCCs, and 13 (87%) of 15 oncocytomas. Among the 81 cases of CRCC, positivity for
S-100 protein was seen in 41 (71%) of 58 and 15 (65%) of 23 cases with Furhman nuclear grade I/II and III/IV, respectively. Focal immunostaining was present in 22 (92%) of 24 normal renal tubules. Similar staining pattern was observed in 21 (70%) of 30 metastatic RCCs. Western blotting demonstrated the
S-100 protein expression in both renal cell
neoplasm and normal renal tissue. Overexpression of S-100 in oncocytomas compared with ChRCCs was confirmed by the data of Western blot and
cDNA microarray analysis. Importantly, 14.8% (12/81) of clear cell RCC and 13.3% (4/30) of metastatic RCC revealed an immunostaining profile of pancytokeratin (-)/
S-100 protein (+). These data indicate that caution should be taken in interpreting an unknown primary with S-100 positivity and
cytokeratin negativity. In addition, it suggests that S-100 has a diagnostic value in differentiating
oncocytoma from ChRCC.