Molecular characterization of helodermin-preferring VIP receptors in SUP T1 lymphoma cells: evidence for receptor glycosylation.

Abstract

Cross-linking of [125I]helodermin to human SUP-T1 lymphoblasts with bis[2-(succinimidooxycarbonyloxy)ethyl]sulfone (BSOCOES) revealed a 63 K binding protein. This cross-linking was inhibited by helodermin and VIP. In cells submitted for 3-4 days to 0.2 microgram/ml tunicamycin, the Mr of an increasing proportion of helodermin-preferring receptors was reduced to 50 K and the total number of receptors was decreased by about 50%, without alteration in binding affinity and specificity. In parallel, the VIP-mediated adenylate cyclase stimulation was reduced by 30% with no change in NaF-, Gpp[NH]p-, and PGE1-stimulations. We conclude that a proper N-glycosylation of helodermin-preferring VIP receptors is required for normal receptor targeting and turnover but not for ligand binding and adenylate cyclase coupling.

• Authors: P Gourlet, P Robberecht, J Christophe
• Journal: Journal of receptor research (J Recept Res) Vol. 11 Issue 5 Pg. 831-48 ( 1991) ISSN: 0197-5110 [Print] United States
• PMID: 1656036 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cross-Linking Reagents
• Intercellular Signaling Peptides and Proteins
• Peptides
• Receptors, Gastrointestinal Hormone
• Receptors, Vasoactive Intestinal Peptide
• Succinimides
• Venoms
• Vasoactive Intestinal Peptide
• bis(2-(succinimidooxycarbonyloxy)ethyl)sulfone
• heliodermin
• Adenylyl Cyclases

Topics

• Adenylyl Cyclases (chemistry)
• Amino Acid Sequence
• Cell Line
• Cross-Linking Reagents
• Enzyme Activation
• Glycosylation
• Humans
• Intercellular Signaling Peptides and Proteins
• Lymphoma (chemistry, enzymology)
• Molecular Sequence Data
• Peptides (chemistry)
• Receptors, Gastrointestinal Hormone (chemistry)
• Receptors, Vasoactive Intestinal Peptide
• Succinimides (chemistry)
• Tumor Cells, Cultured
• Vasoactive Intestinal Peptide (chemistry)
• Venoms (chemistry)