Abstract | BACKGROUND: METHODS: To induce pancreatitis, rats were injected with l-arginine (500 mg/kg) intraperitoneally or saline (control). Pancreatitis was confirmed by measuring serum amylase and evaluating pancreatic histology. Activation of nociceptive pathways was evaluated by counting Fos-like immunoreactive nuclei (FLI) in the dorsal horn of the spinal cord at T3-L1. Some animals received the CGRP antagonist CGRP(8-37) (50 microg intrathecally) 2 hours before perfusion. Animals were compared using a 2-tailed t test. RESULTS:
l-Arginine treatment induced acute necrotizing pancreatitis in the rat at 24 hours. l-Arginine (24 hours) increased FLI in the dorsal horn of the spinal cord, with a peak effect at L1. Intrathecal administration of CGRP(8-37) significantly decreased the number of FLI nuclei in the dorsal horn of the spinal cord in T11-L1. CONCLUSIONS:
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Authors | Elizabeth C Wick, Stella Pikios, Eileen F Grady, Kimberly S Kirkwood |
Journal | Surgery
(Surgery)
Vol. 139
Issue 2
Pg. 197-201
(Feb 2006)
ISSN: 0039-6060 [Print] United States |
PMID | 16455328
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Arginine
- Calcitonin Gene-Related Peptide
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Topics |
- Acute Disease
- Animals
- Arginine
(administration & dosage)
- Calcitonin Gene-Related Peptide
(metabolism, physiology)
- Disease Models, Animal
- Male
- Necrosis
- Pain
(physiopathology)
- Pancreatitis
(complications, physiopathology)
- Posterior Horn Cells
(physiology)
- Rats
- Rats, Sprague-Dawley
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