Protective effect of S-allylcysteine on 3-nitropropionic acid-induced lipid peroxidation and mitochondrial dysfunction in rat brain synaptosomes.

Abstract

3-Nitropropionic acid is a neurotoxin that irreversibly inhibits succinate dehydrogenase, a relevant enzyme constituting the complex II of the respiratory chain during mitochondrial electron transport. 3-Nitropropionic acid is known to produce oxidative/nitrosative stress and evokes an experimental model of Huntington's disease. In this work we evaluated the effects of the antioxidant compound and major organosulfur garlic derivative, S-allylcysteine, on lipid peroxidation and mitochondrial dysfunction induced by 3-nitropropionic acid in synaptosomal fractions from rat brain. 3-Nitropropionic acid, at concentrations ranging 0.75-2.5 mM, produced enhanced levels of lipid peroxidation, while increasing concentrations of S-allylcysteine (0.1-2 mM) decreased the peroxidative action of 3-nitropropionic acid (1 mM) in synaptosomal fractions in a concentration-dependent manner. S-Allylcysteine (0.75 mM) also prevented the 3-nitropropionic acid (1mM)-induced mitochondrial dysfunction. These findings suggest that the protective actions that S-allylcysteine exert on the in vitro neurotoxicity induced by 3-nitropropionic acid are mediated by its antioxidant properties.

• Authors: Verónica Pérez-De La Cruz, Carolina González-Cortés, José Pedraza-Chaverrí, Perla D Maldonado, Leticia Andrés-Martínez, Abel Santamaría
• Journal: Brain research bulletin (Brain Res Bull) Vol. 68 Issue 5 Pg. 379-83 (Jan 30 2006) ISSN: 0361-9230 [Print] United States
• PMID: 16377446 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Neuroprotective Agents
• Nitro Compounds
• Propionates
• Tetrazolium Salts
• Thiobarbituric Acid Reactive Substances
• 4-anisyltetrazolium blue
• S-allylcysteine
• Cysteine
• 3-nitropropionic acid

Topics

• Animals
• Brain (cytology)
• Cysteine (analogs & derivatives, therapeutic use)
• Dose-Response Relationship, Drug
• Drug Interactions
• Lipid Peroxidation (drug effects)
• Male
• Mitochondrial Diseases (chemically induced, metabolism, prevention & control)
• Neuroprotective Agents (therapeutic use)
• Nitro Compounds (pharmacology)
• Propionates (pharmacology)
• Rats
• Rats, Wistar
• Synaptosomes (drug effects)
• Tetrazolium Salts
• Thiobarbituric Acid Reactive Substances (metabolism)